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A highly sensitive post-column reaction detection system was developed for the liquid chromatographic determination of non-reducing carbohydrates. Non-reducing Carbohydrates were oxidized by periodate prior to forming fluorescent compounds by reaction with guanidine in alkaline solution. The calibration graphs were linear between 50 pmol and 10 nmol.

A highly sensitive post-column detection system for reducing carbohydrates in liquid chromatography was developed using guanidine as the reagent. The limit of determination for pentose, 6-deoxyhexose and reducing saccharides was 5 pmol and that for hexose, N-acetylglucosamine and N-acetylneuraminic acid was 10 pmol. The calibration graphs were linear up t o 1 nmol.