Lactate measurement is important in the fields of sports and medicine. Lactate accumulation can seriously affect an athlete’s performance. The most common problem caused by lactate accumulation in athletes is muscle soreness due to excessive exercise. Moreover, from a medical viewpoint, lactate is one of the main prognostic factors of sepsis. Currently, blood sampling is the most common approach to lactate measurement for lactate sensing, and continuous measurement is not available. In this study, a low-cost continuous lactate monitoring system (CLMS) is developed based on a percutaneous microneedle array that uses a three-electrode lactate sensor. The working electrode has an area of 10 mm × 6 mm, including a 3 × 3 array of stainless-steel microneedles. The length, width, and thickness of each needle are 1 mm, 0.44 mm, and 0.03 mm, respectively. The working electrode is then plated with gold, polyaniline, lactate enzyme, Nafion, and Poly(2-hydroxyethyl methacrylate) (poly HEMA). The reference electrode is a 2 × 1 array covered with AgCl, and the counter electrode is a 2 × 1 array plated with gold. The sensor is incorporated into the CLMS and connected to a smartphone application and the cloud. The CLMS was tested on 40 human subjects who rode indoor bicycles, starting at 100 W and increasing in steps of 25 W at intervals of 5 min until exhaustion. The data acquired from the app connected to the CLMS were analyzed to determine the subjects’ lactate response to exercise and the feasibility of assessing exercise performance and training exercise intensity by using the proposed system.
Globally, the proportion of older adults is increasing. Older people face chronic conditions such as sarcopenia and functional decline, which are often associated with disability and frailty. Proteomics assay of potential serum biomarkers of frailty in older adults. Older adults were divided into non-frail and frail groups (n = 6 each; 3 males in each group) in accordance with the Chinese-Canadian Study of Health and Aging Clinical Frailty Scale. Adults were measured for grip power and the 6-min walk test for physical activity, and venous blood was sampled after adults fasted for 8 h. Ultra-high-performance liquid chromatography-tandem mass spectrometry was used for proteomics assay. The groups were compared for levels of biomarkers by t test and Pearson correlation analysis. Non-frail and frail subjects had mean age 77.5±0.4 and 77.7±1.6 years, mean height 160.5±1.3 and 156.6±2.9 cm and mean weight 62.5±1.2 and 62.8±2.9 kg, respectively. Physical activity level was lower for frail than non-frail subjects (grip power: 13.8±0.4 vs 26.1±1.2 kg; 6-min walk test: 215.2±17.2 vs 438.3±17.2 m). Among 226 proteins detected, for 31, serum levels were significantly higher for frail than non-frail subjects; serum levels of Ig kappa chain V-III region WOL, COX7A2, and albumin were lower. The serum levels of ANGT, KG and AT were 2.05-, 1.76- and 2.22-fold lower (all p < 0.05; Figure 1A, 2A and 3A) for non-frail than frail subjects and were highly correlated with grip power (Figure 1B, 2B and 3B). Our study found that ANGT, KG and AT levels are known to increase with aging, so degenerated vascular function might be associated with frailty. In total, 226 proteins were revealed proteomics assay; levels of angiotensinogen (ANGT), kininogen-1 (KG) and antithrombin III (AT) were higher in frail than non-frail subjects (11.26±2.21 vs 5.09±0.74; 18.42±1.36 vs 11.64±1.36; 22.23±1.64 vs 9.52±0.95, respectively, p < 0.05). These 3 factors were highly correlated with grip power (p < 0.05), with higher correlations between grip power and serum levels of ANGT (r = -0.89), KG (r = -0.90), and AT (r = -0.84). In conclusion, this is the first study to demonstrate a serum proteomic profile characteristic of frailty in older adults. Serum ANGT, KG and AT levels could be potential biomarkers for monitoring the development and progression of frailty in older adults.
BackgroundMyostatin negatively regulates muscle growth, and its inhibition by suitable proteins can increase muscle bulk and exercise performance. However, the reference values of serum myostatin in athletes performing strength training are still lacking.MethodsA cross-sectional study recruiting28 male collegiate athletes performing strength training and 29 age-matched normal controls was conducted.The serum concentration of myostatin and insulin-like growth factor 1 (IGF-1), grip strength, and body composition were the main outcome measures. We used regression models to analyze the correlation between serum markers and the physiological parameters. The athlete group had greater height, weight, body mass index (BMI), fat mass percentage, fat-free mass, muscle mass, waist girth, grip strength, and estimated daily energy expenditure.ResultsThe IGF-1 concentration was higher in the athlete group (324 ± 80 vs. 263 ± 134 ng/ml), but the myostatin levels did not differ (12.1 ± 3.7 vs. 12.4 ± 3.5 ng/ml). The reference value for IGF-1 among the healthy young males was 293 ± 114 ng/ml, correlated with age and height; the value for myostatin was 12.3 ± 3.6 ng/ml, correlated negatively with BMI, fat mass percentage, and waist girth after adjustment for age.ConclusionMyostatin level is negatively related to fat percentage, and serum IGF-1 is positively related to height. The reference values could provide a basis for future doping-related study.
The purpose of this study was to investigate the effects of stiffness regulated by landing styles on drop jump performance. Twenty-four male lacrosse athletes performed drop jumps with stiff (ST), self-selected (SS), and soft (SF) landing from a 0.42 m box. Leg stiffness, ground contact time, depth, jump height, maximum ground reaction force (GRF), GRF at the start of the propulsive phase, mean power, peak power, and the reactive strength index (RSI) were calculated. The results showed that jump height and the RSI had strong correlations to power production in all drop jump styles. Power would be a key factor to overall athletic performance. Repeated measures ANOVA showed significant differences (p < 0.05) in all variables among the three styles. Drop jumps with SS landing had comparable jump height to drop jumps with SF landing and power output to drop jumps with ST landing. Drop jumps with ST landing had significantly lower jump height, but higher GRF, power, and the RSI compared to drop jumps with SF landing. In drop jump testing, drop jumps with SS landing should be used if power and jump height were the major concerns; if the RSI was the major concern, drop jumps with ST landing should be used. Training with drop jumps, one of the main objectives should be increasing power output due to its significant correlation to jump height and the RSI in all conditions.
The purpose of this study was to examine the effects of the taping on knee joint kinematics in the frontal and transverse planes. Nine healthy subjects (eight males and one female) volunteered to participate in this study. The Vicon T40 motion analysis system and two force platforms were used to synchronically record the 3-D trajectories of the reflective markers and the ground reaction forces during side hop. Each subject stood on the dominant leg, and jumped on the force platform from side to side between two parallel strips of tape. The knee joint motion data were grouped into two different landing conditions (jumping outward and inward). The results showed that: in the frontal plane, the condition of taping reduced knee valgus angle. In the transverse plane, the results showed significantly greater external tibial rotation angle at toe-off during outward and inward jumping. Therefore, taping could limit the knee angles in the frontal and transverse plane during side jump. That might have contributed in preventing knee ACL injured. Keywords-protective tape; anterior cruciate ligament; knee valgusI.
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