Escherichia coli was grown in a medium containing [1-14C]methionine and [methyl-3H]methionine, and the 3H/14C ratio was determined for each of the ribosomal proteins derived from the 70S ribosome. Evidence indicates that six proteins from the 50S subunit were methylated: L7, L9, Lii, L12, L18, and L33. Methylation of several other 50S proteins (such as Li, L3, L5, etc.) may also occur. The methylated amino acids in protein Lii have been characterized further and found to be predominately e-trimethyllysine. A small amount of a compound tentatively identified as NG, NIG-dimethylarginine was also detected.
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