A polystyrenesulfonic acid resin in the hydrogen form is a useful stationary phase for chromatographicseparation of adenine, caffeine, theobromine, theophylline. tannic acid, xanthine and tea components with aqueous ethanol as eluent. The resulta indicated that caffeine, theophylline, theobromine, adenine, xanthine and tannic acid could be separated on a column of Dowex-50W-X8(H)"~by elution with 25% ethanol aa eluent.
This is to report a study in which cation ion‐exchange resin serves as the stationary phase for liquid chromatography of benzoic acid and phthalic acid and aqueous ethanol acts as the mobile phase. No ion. exchange phenomenon is involved. It is rather a solvent action on the polymer matrix for the organic solutes. It was found that the elution volumes depend on the pKa values of the acids; the higher the pKa value the more the retention volume.
A 8% cross‐linked sulfonated polystyrene cation exchanger in the hydrogen or sodium form was used as stationary phase for the chromatography of aspirin, salicylic acid and acetic acid. The eluent is 0.02 M mineral acids or their sodium salts in aqueous ethanol. Phosphoric acid, nitric acid or sulfuric acid added in aqueous ethanol eluent made successful separation of aspirin, salicylic acid and acetic acid on hydrogen form cation exchanger.
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