Background: SfmD is a hypothetical protein thought to be a hydroxylase in saframycin A biosynthesis. Results: SfmD binds heme and hydroxylates tyrosine analogs using H 2 O 2 as oxidant. Conclusion: SfmD is a heme peroxidase that catalyzes hydroxylation of 3-methyltyrosine to 3-hydroxy-5-methyltyrosine using H 2 O 2 as oxidant. Significance: This study reveals a novel type of heme peroxidase and has significance for the biosynthesis of analogues of saframycin A.
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