The human leukocyte antigen (HLA)-bound-viral antigens serve as an immunological signature that can be selectively recognized by T cells. As viruses evolve by acquiring mutations, it is essential to identify a range of viral presented antigens. Utilizing HLA-peptidomics we were able to identify SARS-CoV-2-derived peptides presented by highly prevalent HLA Class-I (HLA-I) molecules using infected cells as well as overexpression of SARS-CoV-2 genes. We found 26 HLA-I peptides and 36 HLA class-II (HLA-II) peptides. Among the identified peptides some were shared between different cells and some were derived from out-of-frame-ORFs. Seven of these peptides were previously shown to be immunogenic, and we identify two additional immuno-reactive peptides using HLA-multimer staining. These results may aid the development of the next generation of SARS-CoV-2 vaccines based on viral-specific-presented antigens that span several of the viral genes.
and NF are inventors on a patent titled "Identification of recurrent mutated neopeptides" (WO2020/234875) and "TCRS directed at RAS-derived recurrent neoantigens" (Israeli patent application no. 282814).
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