We present a new method for the quantitative determination of high molecular
weight alkaline phosphatase (ALP). This method consists of electrophoretic separation on
polyacrylamide, incubation of the ALP isoenzymes with substrate, gel drying and quantitative
evaluation by densitométrie measurement. This method separates high molecular
weight ALP from the other isoenzymes. Precision of the method is calculated by the coefficient
of variation ranging from 1.2 to 9.5% for samples with different high molecular weight
ALP values. The diagnostic sensitivity for detecting colorectal cancer is 60%.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.