Work is in progress to determine the smallest biologically active moiety in urine, its chemical nature, and whether its primary site of action is bone marrow or some other tissue or organ.Szi~itmary. Urinary erythropoietic factor has been partially purified by adsorption on kaolin followed by serial elution with ammonium acetate buffers of increasing pH and then with 111 KH,OH. L-sing this procedure, a 39% yield with a 230 fold purification of the active factor has been obtained. The purified preparation of the active factor produces metachromasia with toluidine blue, has an absorption peak a t 280 mp, and migrates very slowly on paper strips in an electrophoretic apparatus. The similarity and differences between urinary and plasma erythropoietin are discussed.The view has been expressed that an intimate physiological relationship exists within the circulating blood between fibrin formation and dissolution ( fibrinolytic) mechanisms and the integrity of the blood vessel wall ( 1.2 ) .Previous work (3,4) has presented evidence that the pituitary-adrenal cortex axis exerts a controlling influence on the fibrinolytic (plasmin-antiplasmin) system in the blood. In continuation of studies ( 5 ) on the influence of certain modified corticosteroids, more potent than cortisone, on antitryptic activity of rat serum, 'Medrol ( A1-6-a methylcortisol) and Aristocort diacetate ( 16a-hydroxy-9a-fluoro-cortisol-16a, 2 1 diacetate) were compared with cortisone in normal, hypophysectomized and adrenalectomized rats.Materials and methods. Male Sprague-Dawley rats weighing 150-200 g were used. Sormal and hypophysectomized animals were allowed Purina Laboratory chow and water ad libitum, while adrenalectomized rats were fed Purina Laboratory chow and 0.9% saline ad libitum. All administrations were given intramuscularly; controls received either no injections, or a volume of 0.9% saline equal to that used in the steroid injections. Adrenalectomy and hypophysectomy were performed by guest on July 24, 2015 ebm.sagepub.com Downloaded from
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