Bluegills (Lepomis macrochirus Rafinesque) from two central Tennessee lakes revealed monthly and seasonal variations of liver RNA‐DNA ratios (liver RNA/DNA) and liver‐somatic indexes (LSIˈs) that were associated with gonad maturation, spawning, and temperature. Liver RNA/DNA indicated a longer summer growth depression in fish collected from the smaller, shallower lake. Although LSIˈs were consistently higher in the smaller, shallower lake, liver RNA/DNA and scale analyses showed faster growth rate in the larger, deeper lake. Thus the LSI may be a useful indicator of relative food intake‐energy storage levels within a single lake, but it does not necessarily reflect absolute growth rate of the fish. Liver RNA/DNA provided an indication of immediate or short‐term growth rates.
The dinoflagellate Amyloodlnium ocellatum, which causes amyloodiniosis or 'marine velvet disease', 1s one of the most senous ectoparasitic diseases plaguing warmwater manne fish culture worldwide We report that tomato clownfish Amphlpnon frenatus develop strong immunity to Amyloodlnlum ocellatum infection following repeated nonlethal challenges and that specific ant~bodies are associated with this response React~on of immune fish antisera against dinospore and trophont-denved antigens in Western blots indicated both shared and stage-speclfic antibody-antigen reactions A mannan-binding-protein affinity column was used to isolate IgM-like antibody from A frenatus serum The reduced Ig consisted of one 70 kD heavy chain and one 32 kD light chain with an estimated molecular weight of 816 kD for the native molecule Immunoglobulin (Ig) isolated from immune but not nonunmune fish serum significantly inhlblted parasite infectivity In vitro An enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal rabbit antibody produced a g a~n s t aff~nity-punfied A frenatus Ig Anti-Amyloodlnium serum antibody was not always detectable in immune fish although serum antibody titers in immune fish increased after repeated exposure to the parasite These results suggest that there may be a localized antibody response in skin/gill epithelia1 tissue although antibody was rarely detected in skin mucus
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