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Gonadal differentiation in brook trout Salvelinus fontinalis, as determined from samples collected weekly from 42 to 1,572 degree-days (ЊC) posthatch, occurs between 393 and 464 degree-days posthatch. During this interval, the gonad transforms from a structure containing several developing primordial germ cells into either a gonad with well-developed perinucleolar oocytes or a gonad with cavities that ultimately develop into sperm ducts or the gonadal circulatory system. Brook trout demonstrate differentiated development, in which the gonad develops directly into a testis or an ovary, and anatomical differentiation precedes cytological differentiation in both ovaries and testes. The data provided by this study should aid in the development of techniques for hormonal sex reversal in brook trout.

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