In this study, three-photon absorbing cyclometalated iridium(III) complexes (Ir-H, Ir-8C, and Ir-O) were rational designed and synthesized through fine-tuning of an auxiliary ligand. The long alkoxy chains in Ir-8C and...
Histones
are the alkali proteins in eukaryotic somatic chromatin
cells which constitute the nucleosome structure together with DNA.
Their abnormality is often associated with multiple tumorigenesis
and other human diseases. Nevertheless, a simple and efficient super-resolution
method to visualize histone distribution at the subcellular level
is still unavailable. Herein, a Zn(II) terpyridine complex with rich-electronic
azide units, namely, TpZnA–His, was designed and synthesized.
The initial in vitro and in silico studies suggested that this complex is able to detect histones rapidly
and selectively via charge–charge interactions
with the histone H3 subunit. Its live cell nuclear localization, red-emission
tail, and large Stokes shift allowed super-resolution evaluation of
histone distributions with a clear distinction against nuclear DNA.
We were able to quantitatively conclude three histone morphology alternations
in live cells including condensation, aggregation, and cavity during
activating histone acetylation. This work offers a better understanding
as well as a versatile tool to study histone-involved gene transcription,
signal transduction, and differentiation in cells.
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