Paulownia catalpifolia is an important, fast-growing timber species known for its high density, color and texture. However, few transcriptomic and genetic studies have been conducted in P. catalpifolia. In this study, single-molecule real-time sequencing technology was applied to obtain the full-length transcriptome of P. catalpifolia leaves treated with varying degrees of drought stress. The sequencing data were then used to search for microsatellites, or simple sequence repeats (SSRs). A total of 28.83 Gb data were generated, 25,969 high-quality (HQ) transcripts with an average length of 1624 bp were acquired after removing the redundant reads, and 25,602 HQ transcripts (98.59%) were annotated using public databases. Among the HQ transcripts, 16,722 intact coding sequences, 149 long non-coding RNAs and 179 alternative splicing events were predicted, respectively. A total of 7367 SSR loci were distributed throughout 6293 HQ transcripts, of which 763 complex SSRs and 6604 complete SSRs. The SSR appearance frequency was 28.37%, and the average distribution distance was 5.59 kb. Among the 6604 complete SSR loci, 1–3 nucleotide repeats were dominant, occupying 97.85% of the total SSR loci, of which mono-, di- and tri-nucleotide repeats were 44.68%, 33.86% and 19.31%, respectively. We detected 112 repeat motifs, of which A/T (42.64%), AG/CT (12.22%), GA/TC (9.63%), GAA/TTC (1.57%) and CCA/TGG (1.54%) were most common in mono-, di- and tri-nucleotide repeats, respectively. The length of the repeat SSR motifs was 10–88 bp, and 4997 (75.67%) were ≤ 20 bp. This study provides a novel full-length transcriptome reference for P. catalpifolia and will facilitate the identification of germplasm resources and breeding of new drought-resistant P. catalpifolia varieties.
Alopecia for patients with discoid lupus erythematosus can sometimes be a refractory condition, where mixed infiltrates of T lymphocytes and histiocytes leads to destruction of hair follicles, which might cause permanent scarring. Early diagnosis and timely treatment can achieve hair regeneration and prevent further disease progression. Concentrated growth factor, a novel autologous plasma extract, contains various growth factors that could promote tissue regeneration. In this article, we report a case of cell growth factor combined with corticosteroids for the treatment of discoid lupus erythematosus alopecia. This case study concludes with satisfactory clinical effect.
The wood properties of Paulownia clones determine their ultimate price and uses. This study selected superior clones with good color and mechanical properties using selection indexes. Variation in 23 5-year-old Paulownia clones was analyzed using genetic parameters, correlation analysis, and a comprehensive assessment of two color characteristics [color difference (ΔE) and whiteness (WH)] and six mechanical properties [density (ρ), hardness of the tangential, radial, and end surfaces (Ht, Hr, and He), and cleavage strength of the tangential and radial surfaces (qt, qr)]. There were significant differences (p < 0.01) in each of the eight traits among the 23 clones. There were significant negative phenotypic and genetic correlations between ΔE and WH. The six mechanical properties were significantly positively correlated genetically, showing significant positive phenotypic correlations with each other, except for ρ, Ht, and qt. With a selection rate of 8.70%, clones MB04 and L01 were selected as superior using the comprehensive selection index. Compared with the control (9501), the genetic gains of clones MB04 and L01 in ΔE, WH, ρ, qr, qt, He, Hr, and Ht were 0.40, 0.21, 10.32, 12.57, 14.81, 26.05, 28.04, and 6.84%, respectively, and the actual gains were 0.59, 0.31, 17.21, 28.45, 28.09, 34.90, 40.08, and 11.12%.
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