Microbially Induced Calcite Precipitation (MICP) has grown up to be a hot topic in geotechnical engineering. MICP is a technology that controls and utilizes microbial reactions to produce calcium carbonate cement. It can be used to solve problems in geotechnical engineering, such as improving rock and soil strength, reducing permeability, treating the polluted soil. Microbial mineralization involves a series of biochemical reactions, and the curing process is of particular importance. Therefore, the curing effect of MICP is restricted and impacted by many factors. Built on the factors of microbial mineralized calcium carbonate reaction and microbial mineralization, the principles of urea hydrolysis, denitrification and sulfur reduction were summarized. The effects of air conditions, environmental factors, fiber addition amount, soil water content, and treatment solution concentration on microbial mineralization technology were analyzed. The conclusion is as follows: in the aerated state, the calcium carbonate content is the highest; the low urease concentration, the natural climatic temperature (the best in summer), the pH value between 7-9 produces the most calcium carbonate and the compressive strength is the largest; When soil fiber content is 3%-5%, MICP technology works best; the low concentration treatment liquid is more suitable for the MICP technology; the microbial mineralization technology is suitable for the sand, but it can also produce good effects for other complex soil.
Urea hydrolysis is widely used in agriculture, environment and other engineering fields, among which urease contained in beans can catalyze urea hydrolysis. The urea hydrolysis activity of legume plant leaching solution (LPLS) was investigated, including soybeans, black beans, mung beans, red beans as well as soybean hulls, soybean leaves, soybean stems and soybean pods. For the high urea hydrolysis activity and economic efficiency, soybean is most suitable for agricultural engineering and other fields than other beans and soybean-related parts extract. The urea hydrolysis activity increases with the concentration of LPLS, while decreases gradually with reaction time. When the heating temperature reaches 25, 35, 45, 55 and 65 ℃, the urea hydrolysis activity is steady and the enzyme activity is high. Enzyme activity decreases after 65 °C (i.e.75, 90 °C). Meanwhile, the soaking time of LPLS has a little effect on the urea hydrolysis activity compared with other factors. These results make a positive contribution to domestic production urease experimental basis.
Urea hydrolysis is widely used in agriculture, environment and other engineering fields, among which urease contained in beans can catalyze urea hydrolysis. The urea hydrolysis activity of legume plant leaching solution(LPLS) was investigated, including soybeans, black beans, mung beans, red beans as well as soybean hulls, soybean leaves, soybean stems and soybean pods. For the high urea hydrolysis activity and economic efficiency, soybean is most suitable for agricultural engineering and other fields than other beans and soybean-related parts extract. The urea hydrolysis activity increases with the concentration of LPLS, while decreases gradually with reaction time. When the heating temperature reaches 25, 35, 45, 55 and 65 , the urea hydrolysis activity is steady and the enzyme activity is high. Enzyme activity decreases after 65°C (i.e.75, 90°C). Meanwhile, the soaking time of LPLS has a little effect on the urea hydrolysis activity compared with other factors. These results make a positive contribution to domestic production urease experimental basis.
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