No abstract

A series of novel bis-indole compounds, 1,omega-bis(((3-acetamino-5-methoxy-2-methylindole)-2-methylene)phenoxy)alkane, have been designed and synthesized on the basis of the enzyme structure of human nonpancreatic secretory phospholipase A2 (hnps PLA2). Their inhibition activities against hnps PLA2 were improved compared to that of the monofunctional protocompound. These bivalent ligands not only inhibited hnps PLA2 but also drove the dimerization of hnps PLA2. Their dimerization ability correlated with the linker length and position. Further study on the potent compound 5 (1,5-bis(((3-acetamino-5-methoxy-2-methylindole)-2-methylene)phenoxy)pentane, IC50 = 24 nM) revealed that cooperative binding interactions between the two enzyme molecules also contributed to the stability of the ternary complex. The combination of bivalent ligands and hnps PLA2 can be used as a novel chemically induced dimerization (CID) system for designing regulatory inhibitors.

We report a unique triple-frequency symmetric subtraction scheme to effectively remove the nonresonant background in coherent anti-Stokes Raman scattering (CARS) microscopy. Theoretical and experimental studies show that this unique scheme has an optimal performance for high contrast vibrational imaging, particularly useful when the resonant signal was larger than or comparable to the nonresonant background.

In order to eliminate zeroth order effect and to make the phase shifting algorithm insensitive to phase shifting error, an 11-frame phase shifting algorithm is proposed in this paper. The analytical expression of phase-restoration error function is derived. The principle of phase shifting error compensation and the capability of suppressing zeroth order effect are explained, in comparison of existing algorithm. The analytical results show that this algorithm's phase-restoration error is proportional to sine of double shearing phase and to biquadratic of phase shifting error. Finally, we generate the interference patterns of 11-frame algorithm and existing algorithm, restore the shearing phases and calculate the phase-restoration errors by simulations. The simulation results verify the theoretical analyses.