Examining tropical regional precipitation anomalies under global warming in 10 coupled global climate models, several mechanisms are consistently found. The tendency of rainfall to increase in convergence zones with large climatological precipitation and to decrease in subsidence regions—the rich-get-richer mechanism—has previously been examined in different approximations by Chou and Neelin, and Held and Soden. The effect of increased moisture transported by the mean circulation (the “direct moisture effect” or “thermodynamic component” in respective terminology) is relatively robust, while dynamic feedback is poorly understood and differs among models. The argument outlined states that the thermodynamic component should be a good approximation for large-scale averages; this is confirmed for averages across convection zones and descent regions, respectively. Within the convergence zones, however, dynamic feedback can substantially increase or decrease precipitation anomalies. Regions of negative precipitation anomalies within the convergence zones are associated with local weakening of ascent, and some of these exhibit horizontal dry advection associated with the “upped-ante” mechanism. Regions of increased ascent have strong positive precipitation anomalies enhanced by moisture convergence. This dynamic feedback is consistent with reduced gross moist stability due to increased moisture not being entirely compensated by effects of tropospheric warming and a vertical extent of convection. Regions of reduced ascent with positive precipitation anomalies are on average associated with changes in the vertical structure of vertical velocity, which extends to higher levels. This yields an increase in the gross moist stability that opposes ascent. The reductions in ascent associated with gross moist stability and upped-ante effects, respectively, combine to yield reduced ascent averaged across the convergence zones. Over climatological subsidence regions, positive precipitation anomalies can be associated with a convergence zone shift induced locally by anomalous heat flux from the ocean. Negative precipitation anomalies have a contribution from the thermodynamic component but can be enhanced or reduced by changes in the vertical velocity. Regions of enhanced subsidence are associated with an increased outgoing longwave radiation or horizontal cold convection. Reductions of subsidence are associated with changes of the vertical profile of vertical velocity, increasing gross moist stability.
Global warming mechanisms that cause changes in frequency and intensity of precipitation in the tropics are examined in climate model simulations. Under global warming, tropical precipitation tends to be more frequent and intense for heavy precipitation but becomes less frequent and weaker for light precipitation. Changes in precipitation frequency and intensity are both controlled by thermodynamic and dynamic components. The thermodynamic component is induced by changes in atmospheric water vapor, while the dynamic component is associated with changes in vertical motion. A set of equations is derived to estimate both thermodynamic and dynamic contributions to changes in frequency and intensity of precipitation, especially for heavy precipitation. In the thermodynamic contribution, increased water vapor reduces the magnitude of the required vertical motion to generate the same strength of precipitation, so precipitation frequency increases. Increased water vapor also intensifies precipitation due to the enhancement of water vapor availability in the atmosphere. In the dynamic contribution, the more stable atmosphere tends to reduce the frequency and intensity of precipitation, except for the heaviest precipitation. The dynamic component strengthens the heaviest precipitation in most climate model simulations, possibly due to a positive convective feedback.
The goal of this study was to establish mini-swine as a large animal model for stem cell-based pulp regeneration studies. Swine dental pulp stem cells (sDPSCs) were isolated from mini-swine and characterized in vitro. For in vivo studies, we first employed both ectopic and semi-orthotopic study models using severe combined immunodeficiency mice. One is hydroxyapatite-tricalcium phosphate (HA/TCP) model for pulp-dentin complex formation, and the other is tooth fragment model for complete pulp regeneration with new dentin depositing along the canal walls. We found that sDPSCs are similar to their human counterparts exhibiting mesenchymal stem cell characteristics with ability to form colony forming unit-fibroblastic and odontogenic differentiation potential. sDPSCs formed pulp-dentin complex in the HA/TCP model and showed pulp regeneration capacity in the tooth fragment model. We then tested orthotopic pulp regeneration on mini-swine including the use of multi-rooted teeth. Using autologous sDPSCs carried by hydrogel and transplanted into the mini-swine root canal space, we observed regeneration of vascularized pulp-like tissue with a layer of newly deposited dentin-like (rD) tissue or osteodentin along the canal walls. In some cases, dentin bridge-like structure was observed. Immunohistochemical analysis detected the expression of nestin, dentin sialophosphoprotein, dentin matrix protein 1, and bone sialoprotein in odontoblast-like cells lining against the produced rD. We also tested the use of allogeneic sDPSCs for the same procedures. Similar findings were observed in allogeneic transplantation. This study is the first to show an establishment of mini-swine as a suitable large animal model utilizing multi-rooted teeth for further cell-based pulp regeneration studies.
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