The methanol extracts of Loranthus micranthus fractions were collected from column chromatography. From TLC, the fraction 17 and 20 were selected based on the clear distiguishable of bands and few phytochemicals. Same fractions when subjected to GC-MS, fraction-17 yielded 13 and fraction-20 yielded 11 prominent compounds. Diabetic rats that were treated with the fraction 17 and 20 showed significantly increase in body weight (16.52 and 12.43 % respectively). Hypoglycemic effect and antihyperlipidemic characters (decrease in the previously elevated levels of serum cholesterol and serum triglycerides) were observed. The two fractions treated rats have also showed the regeneration of pancreatic connective tissue, acini, blood vessels, blood capillaries, pancreatic ducts and their walls, islets capsule and cells in accordance to proper cytoarchitecture. The in vivo antidiabetic property may be due to the presence of single or all phytochemicals in the fraction. Greater activity was observed in fraction-17. However, further experimental studies are still needed for identification of the functionally relevant phytochemical(s) in management of diabetes in rats.sp. were the common host plants of L. micranthus [10]. Herbals are the choice over synthetic drugs from past centuries having multiple therapeutic functions and fewer side effects. In this context it was an effort to attend towards antidibetic studies of L. micranthus in an analytical and systematic ways. L. micranthus has been widely used in ethnomedicine for various purposes, including antihypertensive, anticancer, antispasmodic, and antidiabetic, in treatment of epilepsy, headache, infertility, menopausal syndrome and rheumatism [11,12] and many of medicinal activities are proven by recent researches. It decreases the blood glucose level and controls the loss of body weight in diabetes mellitus [13].Presently the study was aimed to carry out GS-MS and in vivo antidiabetic activities of Loranthus micranthus on alloxan induced diabetic rats. Based on our earlier studies of qualitative, quantitative and some biological activities, column fractions from methanol extracts of L. micranthus were selected for in vivo antidiabetic studies on rats. Body weight, blood glucose level, lipid profile and histopathological changes of rats were considered in the study. Materials and Methods Collection and processing of plantThe fresh leaves of Loranthus micranthus growing on the host plant
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