The study was designed to observe the effects of 50 Hz homogeneous ferromagnetic field (0.097T) exposure on change of intracellular Calcium-ion concentration of human low differentiation gastric adenocarcinoma cell line - SNU and lymphocytes in vitro. SNU cells and human peripheral blood lymphocytes in vitro were exposured to 50 Hz homogeneous ferromagnetic field at different time periods. Cells were harvested after magnetic field exposure. The concentration of intracellular Calcium-ion of cells in control and different experimental groups were measured with confocal microscope. The concentration of intracellular Calcium-ion of SNU cells and human peripheral blood lymphocytes were all increased after magnetic field exposure. 50Hz homogeneous ferromagnetic field exposure could induce increases of the concentration of intracellular Calcium-ion of SNU cells and human peripheral blood lymphocytes in vitro.
We investigate the effects of magnetic field on expression of Bax, Bcl-2 and Caspase-3 of SNU (human low differentiated gastric adenocarcinoma cell line) in this article. SNU cells were randomly divided into control groups and four treatment groups, the treatment groups were respectively irradiated by 50Hz homogeneous ferromagnetic field (0.097T) 5min, 30min, 60min and 120min. SNU cells were harvested for detection of Bax, Bcl-2 and Caspase-3 antigen with flow cytometric analysis (FCM), immunocytochemical staining and Western blot. The expression of Bax and Caspase-3 in all magnetic field exposure groups were higher than that in control by FCM, immunocytochemical stain and Western blot, but the expression of Bcl-2 in all magnetic field exposure groups were lower than that in control by FCM, immunocytochemical stain and Western blot. We concluded that possible mechanisms of apoptosis of SNU cells after 50Hz homogeneous ferromagnetic field (0.097T) treatment may be Up-regulating the expression of Bax and Caspase-3, dowm-regulating the expression of Bcl-2.
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