A comparative analysis on five edible oils with the same chemical composition but different composition ratios was performed by using coherent anti‐Stokes Raman scattering spectroscopy in the wavenumber 2400–3200 cm−1 corresponding to C─H vibration band. Intensity ratio (I═C ─ H/IC ─ H) in C─H stretching region was obtained according to the mixing ratio of the two types of edible oil, and the fitting curves were obtained from the fact that the intensity ratio is proportional to the square of N═normalC─normalHtrue/NnormalC─normalH where Nk is the expectation value of k bonds of the edible oil. N═normalC─normalH and NnormalC─normalH of the edible oils obtained from the fitting curves were well matched with the referred values. And finally, we showed that the coherent anti‐Stokes Raman scattering spectroscopy can be used as a method of authentication of adulterated edible oil by demonstrating a method to determine the type and the amount of unknown mixed oil.

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By utilizing a multimodal nonlinear optical system that combines coherent anti-Stokes Raman scattering and second harmonic generation to investigate biological characteristics of dermal tissues ex vivo, we demonstrate the potential feasibility of using this optical approach as a powerful new investigative tool for future biomedical research. For this study, our optical system was utilized for the first time to analyze lipid and collagen profiles in cereblon knockout (KO) mouse skin, and we were able to discover significant alterations in the number of carbon-carbon double bonds (wild-type vs. cereblon KO; N C C : 0.75 vs. 0.85) of skin fatty acids in triacylglycerides as well as changes in dermal collagen fibers (25% reduction in cereblon KO). By adopting our optical system to biological studies, we provide researchers with another diagnostic approach to validate their experimental results, which will significantly advance the state of biomedical research.

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