Liver is an important metabolic organ with numerous functions in human body. Hepatitis is defined as the inflammation of the liver tissue, which could lead to acute liver failure, liver fibrosis, liver cirrhosis and hepatocellular carcinoma. Corydalis tomentella Franch., a precious herb in China, is often used in the treatment of hepatitis, liver cirrhosis and liver cancer. In this study, 41 isoquinoline alkaloids and derivatives isolated by our lab from C. tomentella and 61 related targets were analyzed by network pharmacology. Their activities were further verified by cell assay evaluated for antitumor activity against HepG2 cells and molecular docking. The results confirmed that the alkaloids from C.tomentella had extensive hepatoprotective effects, and TNF‐α was the key target of hendersinate B methyl ester against acute liver damage by viral hepatitis and HCC, which provided a foundation for further in vivo studies.
A new amide (1), two new phenylpropanoid derivatives (2, 3), along with three new natural products, including three nitrogen chirality compounds, N‐(3‐methoxy‐1,3‐dioxopropyl)‐D‐phenylalanine methyl ester (4), N‐(3‐methoxy‐1,3‐dioxopropyl)‐L‐phenylalanine methyl ester (5), and N‐acetyl‐L‐phenylalanine methyl ester (6), as well as dimethyl (2R,3R)‐2‐hydroxy‐3‐(((E)‐3‐(4‐hydroxyphenyl)acryloyl)oxy)succinate (7) and dimethyl (S,E)‐2‐((3‐(4‐hydroxy‐3‐methoxyphenyl)acryloyl)oxy)succinate (8) were isolated from Delphinium kamaonense Hunth. Their structures were elucidated by extensive analysis of 1D and 2D NMR, and HR‐ESI‐MS experiments, and the absolute configurations were determined by comparative analysis of specific optical rotation. Compound 1 exhibited a moderate cytotoxicity effect against Hep‐3B cancer cell lines (IC50 41.39±0.13 μM) and an excellent antioxidant activity (IC50 0.527±0.06 μM in ABTS assay, and 1.235±0.09 μM in DPPH assay, respectively), which was superior to vitamin C in ABTS (IC50 1.670±0.07 μM) and DPPH (IC50 19.10±0.40 μM) methods.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.