A Gram-staining negative, motile, non-spore-forming, short rod-shaped (0.8-1.5¾1.5-2.0 mm), halophilic bacterium, designated strain NTU-107 T , was isolated from brine samples collected from the abandoned Beimen saltern in southern Taiwan. The novel strain grew with 0-15 % (w/v) NaCl (optimum between 5 % and 10 %), at 15-55 6C (optimum 40 6C) and at pH 5.5-9.5 (optimum pH 7.5). The major cellular fatty acids were C 18 : 1 v7c, C 16 : 0 and C 19 : 0 cyclo v8c, the genomic DNA G+C content was 66.5 mol%, and the predominant ubiquinone was Q-9. The major polar lipids included phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. In a phylogenetic analysis based on 16S rRNA gene sequences, strain T clustered with members of the genus Halomonas. In hybridization experiments, however, the levels of DNA-DNA relatedness between strain NTU-107 T and the type strains of its closest phylogenetic neighbours (Halomonas koreensis, H. organivorans and H. ventosae) were all found to be less than 40 %. Based on the phenotypic, chemotaxonomic and genetic data, strain NTU-107 T represents a novel species within the genus Halomonas, for which the name Halomonas beimenensis sp. nov. is proposed. The type strain is NTU-107 T (5BCRC 17999At the time of writing, the family Halomonadaceae (Franzmann et al., 1988), which belongs to the class Gammaproteobacteria, comprises nine genera with names that have been validly published: Halomonas, Aidingimonas, Chromohalobacter, Cobetia, Carnimonas, Halotalea, Modicisalibacter, Kushneria and Zymobacter (Ventosa et al., 1989;Okamoto et al., 1993; Dobson & Franzmann, 1996;Garriga et al., 1998;Arahal et al., 2002; Ben Ali Gam et al., 2007;Ntougias et al., 2007). The genus Halomonas was originally proposed, with Halomonas elongata as the sole species, to accommodate a group of extremely halotolerant strains (Vreeland et al., 1980 Martínez-Cánovas et al., 2004). Their principal fatty acids are C 16 : 1 cis9, C 16 : 0 , C 17 : 0 cyclo, C 18 : 1 and C 19 : 0 cyclo 11-12, and the major isoprenoid quinone is ubiquinone-9 (Q-9) (Franzmann & Tindall, 1990). Some members of the genus Halomonas have potential use in biotechnology (Margesin & Schinner, 2001;Ventosa & Nieto, 1995), such as in the production of hydrolytic enzymes (Sánchez-Porro et al., 2003) and the degrading of aromatic compounds (García et al., 2004 ): 5 g yeast extract (Difco), 5 g Casamino acids (Difco) and 5 g MgSO 4 . 7H 2 O. The pH of the basal medium agar was adjusted to pH 7.0 with 1 M NaOH. The plates were incubated at 37 u C for 7 days. Anaerobic growth was examined by incubating plates in an anaerobic chest with an AnaeroPack (Mitsubishi Gas Chemical). After strain NTU-107 T was isolated, its salt tolerance was tested in liquid basal medium containing 0-30 % (w/v) NaCl. Liquid basal medium was also used to determine the novel strain's temperature range for growth (by incubating at temperatures between 10 and 70 u C) and its pH range for growth (by incubating in medium that had been adjusted, with 0.1 M NaOH or ...