Abstract. As a key molecule involved in cell recognition, calreticulin (CRT) may be expressed on the surface of (pre-) apoptotic cells and provide the signal that is recognized by dendritic cells (DCs) or other antigen presenting cells (APCs), which results in phagocytosis. Within the APCs, tumor-associated antigens (TAAs) may be subsequently presented to T lymphocytes, which triggers a specific antitumor immune response. It has been hypothesized that CRT is able to act as the immunologic adjuvant and translocate itself and TAAs to the cell surface and induce a specific antitumor immune response. In the present study, CRT was demonstrated to translocate itself and mucin 1 (MUC1), a breast cancer antigen, to the surface of 4T1 cells and the MUC1-CRT-coated cells were able to induce apoptosis in a time-dependent manner. When DCs were infected with adenovirus containing MUC1-CRT, an increase in T cell proliferation and cytokine production was exhibited. These results suggest that CRT may act as an immunologic adjuvant with MUC1 and induce a strong immune response.
Electro-thermal analysis of a new micro-hotplate designed for gas sensor has been studied in this paper. Through the comparison of materials, select pt as electrodes material, Si, SiO2 as substrate materials. Then, through the optimization of substrate structure, in order to make the gas sensor obtain perfect properties of good temperature uniformity, the thickness of Si substrate, thermal insulation layers are 150, 50, 100 µm respectively. The new micro-hotplate structure is benefit for the improvement of sensitivity and overall performance of the sensor.
A new kind of electrode was designed for micro-structural gas sensor based on silicon substrate. The temperature distribution was simulated by means of finite element analysis tool ANSYS, in order to obtain higher temperature and uniform temperature distribution in the micro-gas sensor, the thickness of SiO2, thickness of Si substrate, electrode width and electrode space is designed to be 50, 250, 10 and 350 µm, respectively. The magnetic field distribution was also analyzed by ANSYS, and the results show that the new electrodes can eliminate the magnetic field from the heater on the measurement signal interference, which is benefit for the improvement of sensor performance.
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