The current investigation is intended to evaluate the content of phytochemical constituents and antioxidant potential of hydroalcoholic extracts of stem and root of Grewia serrulata DC (HAESGS & HAERGS) and leaf and bark of Grewia Nervosa (Lour.) panigrahi (HAELGN & HAEBGN). Initially, all the extracts at different concentrations were estimated for their total phenolic content and total flavonoid content. The study was further extended for their antioxidant potential evaluation using various in vitro methods such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical scavenging assays. The total phenolic content (mg gallic acid equivalent per gram of extract) was high in HAELGN (170.82±0.19) and HAERGS (123.00±0.48) than HAESGS (111.2±0.26) and HAEBGN (119.60±0.23). The total flavonoid content (mg quercetin equivalent per gram) is greater in HAERGS (71.24±0.50) and HAESGS (65.68±0.27) than HAELGN (55.82±0.35) and HAEBGN (62.38±0.45). The IC50 values (µg/ml) of different plant extracts inferred that DPPH radical scavenging activity is greater in HAELGN (42.91±0.88) and HAEBGN (53.87±0.35) than HAESGS (126.73±1.20) and HAERGS (88.87±1.25). However, hydroxyl and superoxide radical scavenging activity is more in HAERGS (135.41±1.19 & 88.00±1.42) and HAELGN (172.28±1.91 & 108.163±1.09) than HAESGS (237.3±1.65 & 110.074±0.87) and HAEBGN (204.7±1.04 & 125.54±1.07). The results of present comprehensive analysis demonstrated that both the plants Grewia serrulata DC and Grewia Nervosa (Lour.) panigrahi possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a valid source of natural antioxidants and might be utilized for pharmacological screening of various therapeutic activities.
Keywords: Grewia serrulata; Grewia Nervosa; Total Phenolic content; Total flavonoid content; Antioxidant potential
Abstract. Prescription auditing, a sort of vigilance activity is an important tool in pharmaco-epidemiological studies to get a clear picture about rational usage of drug, widely prescribed drugs, extent of polypharmacy, and the most prevalent diseases in a particular area. With the changing dynamics of the lifestyle, behavioral pattern, socioeconomic and educational status an increasing trend of self medication observed in both rural and urban dwellers. Till yet there is no such report about the drug utilization pattern or extent of self medication practice in the Kharaghpur area. A study was conducted in Kharaghpur region of India (Block 1 and 2) to determine the drug utilization trend and practice of self medication in the prevailing disease conditions by prescription monitoring and exit interviews with pretested Performa on customers randomly visiting the pharmacies. Prescription auditing showed that disease prevalence was predominant in age group of 11.00±6.2550.00±9.75 and comparatively less incidences in the pediatrics (1.00±1.055.00±0.79) and geriatrics (70.00±1.3175.00±1.01). There was a predominance of infectious diseases like fever, cough and cold during the study period followed by asthmatic problems and gastrointestinal infections GIT infections in the surveyed area. Self-medication of asthma and GIT infections was more prevalent in urban population (60%, n=133) as compared to the rural population (33.89%, n=107). Easy availability of lifestyle drugs, enhanced education levels amongst urban population and economic hindrance to pay physicians fees, influences of peer groups, advices of pharmacists, difficulty to avail drugs from clinics in rural regions influenced self medication. Analgesics, antipyretics were the most widely used self medicating drugs in survey area.
The objective of this study was to formulate and optimize a stable rilpivirine nanosuspension. In the present study, yttrium stabilized zirconium oxide beads being used as the milling media in nanomilling process. The lyophilized nanocrystals were being characterized by particle size distribution (PSD), polydispersity index (PDI), X-ray diffraction (XRD) and FTIR (Fourier transform infrared spectroscopy). Optimized nanosuspension has mean particle diameter of 266 nm, PDI of 0.158, zeta potential of 22.1 mV and spherical in shape with surface oriented stabilizer molecules. Flow properties like sedimentation volume, poura-bility with the F value of 0.94 and also the redispersability even after 4 weeks of storage was found to be satisfactory for the optimized nano-suspension. Many folds increase in solubility and rate of drug release observed, The lyophilized nanocrystals retains its crystallinity after nanomilling, stable chemically with high drug content, therefore, the developed nanosuspension would be an alternative better formulation than its conventional formulation to address its bioavailability issue. However, this should be further confirmed by appropriate techniques in vivo studies.
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