BackgroundOral and pharyngeal cancer (OPC) of multifactorial aetiology is a major health problem globally. Ranking first in all cancers, OPC poses a significant impact on the Sri Lankan male population. As Human Papillomavirus (HPV) high risk (HR) types are found to be significant risk factors for OPC globally, the current study was undertaken to examine the association between HR-HPV16 and 18 types with OPC in Sri Lanka.Materials and methodsSerum samples of 78 OPC patients and 51 non-cancer controls were assayed for the presence of anti-HPV16 and anti-HPV18 IgG antibodies using in-house established Enzyme Linked Immunosorbent Assays (ELISAs). The association between OPC and its risk factors i.e. HPV, smoking, alcohol, betel quid, poor dentition, was established using Chi-square test. Logistic regression was used to calculate odds ratios (OR), adjusted for the influence of other risk factors.ResultsThis prototype study in Sri Lanka showed a significant risk of 15 fold in developing OPC due to HPV16/18 seropositivity after removing variability due to other factors. Oncogenic HPV18 showed a higher rate of seropositivity being detected in 32% of OPC patients, and also in 2% of non-cancer control subjects. HR-HPV16 was detected in 23% of OPC patients and in 5.88% of controls. Moreover, seven OPC patients were detected with both anti-HPV16 and anti-HPV18 antibodies. According to the logistic regression models HPV18 seropositivity was associated with a 28 fold risk in developing OPC while that of HPV16 was associated with a 6 fold increase in risk for the development of OPC. A 5 fold risk of developing OPC was also pronounced among smokers while alcohol, betel and poor dentition was not significantly associated with OPC. Statistically significant differences with regard to age, gender, smoking, alcohol, betel use, poor dentition and site specificity of the tumour was not observed between HPV seropositive and seronegative OPC patients.ConclusionsBoth in-house developed ELISAs detected significant proportions of HPV seropositives within the OPC study population suggestive of HPV as a strong risk factor for oral and pharyngeal carcinogenesis in Sri Lanka.
Inflammation contributes to knee osteoarthritis (KOA) where many immunological mediators participate in its initiation and progression. Most clinicians manage primary (pKOA) and secondary osteoarthritis (sKOA) alike. Whether immunological profiles of pKOA and sKOA differ remains obscure. Hence, we aimed to differentially identify potential serum immunologic diagnostic markers of pKOA and of sKOA. This case control study used 46 KOA patients (pKOA, n = 30; sKOA, n = 16), and 60 age, gender matched controls (normal healthy, n = 30; systemic lupus erythematosus [SLE] disease controls, n = 30) where serum was assayed for cytokines (TNF-α, IL-1β, IL-6, IL-10) and nitric oxide derivatives (NOx). Sandwich ELISA assessed cytokine levels, while the ‘Griess assay’ quantified NOx levels. The diagnostic accuracy of optimal marker combinations was evaluated by the CombiROC web tool. Compared with pKOA, sKOA serum displayed significantly elevated levels of pro inflammatory cytokines (TNF-α, IL-1β, IL-6) with a concurrent decrease in the anti-inflammatory cytokine, IL-10 (P<0.05). This was reiterated by significantly higher Th1:Th2 (TNF-α: IL-10) serum cytokine ratio observed in sKOA compared to that of pKOA. The CombiROC curves identified TNF-α, IL-1β, IL-6 and NOx as the best performing panel of potential diagnostic markers to discriminate pKOA from control groups (~97% accuracy, 90% Sensitivity [SE] and 98% specificity [SP]), while TNF-α, IL-1β and IL-6 discriminated sKOA from control groups (~100% accuracy, 100% SE, and 98% SP). The study identified discrete serum immune biomarker panels to differentiate between pKOA (TNF-α, IL-1β, IL-6 and NOx) and sKOA (TNF-α, IL-1β and IL-6). These findings may assist in developing distinct therapeutic agents for the two types of KOA.
Seminal oxidative stress plays an important role in male factor infertility (MFI), worldwide. A study was thus undertaken for the first time to establish seminal reactive oxygen species (ROS) as a clinical marker of MFI in a cohort of Sri Lankan males. The nitro blue tetrazolium (NBT) assay for ROS estimation and modified Endtz test for detecting leucocytes were carried out on semen samples (N = 102) of subfertile males. Age-matched individuals (N = 30) with proven past paternity served as controls. Significantly higher ROS production was evident in individuals with asthenozoospermia and unexplained infertility (Mann-Whitney U-test, P = 0.000), than in the fertile and the other subfertile groups tested. Receiver operating characteristic plot analysis established cut-off points of 40.57 and 42.02 μg formazan/10(7) spermatozoa for ROS to distinguish fertile males from asthenozoospermics (71.4% sensitivity: 70% specificity; AUC = 0.82), and from unexplained infertile males (74.1 % sensitivity: 73.3% specificity; AUC = 0.85) respectively. As ROS appear to be a potential marker of male infertility, it is imperative to validate this test as a simple, cost-effective hence a widely accessible diagnostic tool to be included in MFI investigations in the developing world.
Purpose Higher educational institutes (HEIs) are experiencing a significant shift towards online education, which has been fast-forwarded with the global pandemic of COVID-19. The forced shift has also exposed many vulnerabilities in online education, especially assessments. The purpose of this study is to investigate the potential dark side of the digital transformation of examinations through the lens of university students. Design/methodology/approach This study involves a sample of 127 university students from the fields of business and science, technology, education and management (STEM) and the key factors affecting student perception were assessed quantitatively to explore the interrelationships. Findings Results revealed that both business and STEM students have a similar impression of the use of online examinations, and the majority still have mixed feelings about them as a replacement for physical examinations. The regrouping of the factors revealed two key dimensions, trustworthiness and apprehensible education, as key areas of student perception in the context of online examinations. Research limitations/implications This study aims to strengthen the understanding of Kolb’s experiential learning mechanism through a discussion on the importance of abstract conceptualization as opposed to concrete experience in the establishment of the online assessment and learning space. Practically speaking, increasing investment in internet infrastructure and forming strategic alliances with important parties, like internet providers, to create uninterrupted network coverage, are an effective place to start if one wants to make sure that the process of moving to online learning is becoming more and more accepted by educators, students, and the general public. Originality/value The online transition to higher education has seen expedited growth since the pandemic and has not given much room for many HEIs globally to adjust. The procedures and techniques implemented take a Western lens, and less attention is given to the emerging context and its context-specific characteristics in such implementation. This study takes the theoretical lens of Kolb and proposes the key learnings for a successful online transition to assessment in emerging contexts.
The paper aims to explore the key policy devising measures under crisis management in dealing with the COVID-19 pandemic. A key argument in the study is that building policy for crisis requires the understanding of both governance capacity as well as legitimacy. Through the theoretical lens of crisis management and resilience building, the study explores the key literature on the topic to arrive at a conceptual framework which is further explored through mixed-method research. The study explores the phenomenon in the context of Sri Lanka and its mechanism to deal with the pandemic. Fifteen interviews were conducted, followed by a survey covering key sectors of tourism, information technology and education to understand the extent to which crisis management strategies were achieved in the policy procedures. The article identifies some key themes: political capacity, stakeholder involvement, input capacity and throughput capacity in the overall governance system that requires further improvements, including the gender differences in accepting the policy decisions, which can be explored in future work for greater insight.
Vision-impaired students have several issues accessing mathematical documents, which are associated with the representation of mathematical expressions and graphs of mathematical functions. Graphs of mathematical functions are visual presentations of mathematical information and useful to illustrate numerical or qualitative information, which are sometimes difficult or even impossible to describe. A graph is a picture that shows how sets of data are related to each other. Graphs of mathematical functions generally convey the intended message of a mathematical document. Therefore, access to graphs is essential in learning mathematics. Finding a solution to represent graphs of mathematical functions in an accessible format is necessary for the vision-impaired. This paper describes an approach to develop an application to address this issue by detecting, extracting and categorising important information from graphs of mathematical functions using three open source packages, namely, ImageMagick, GNUPLOT and Octave.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.