In order to meet the demand for medicinal sage (Salvia officinalis L.), which is an important economic product, harvesting from nature has economic value. However, it may not always be of the desired standard and quality. Also, the harvesting from nature endangers their natural population causing their genetic base to decline. For this reason, it is important to produce it in an agrosystem and to increase yield in a sustainable way. In this study, the effects of eleven endophyte bacteria (EB) isolates applications on the development, morphology, and physicochemical properties of Salvia officinalis L. were investigated by climate chamber experiments. Peat+perlite+soil (1:1:2) mixture was used as the growing medium and EB was applied two times by soaking method. Effects of EB applications on shoot/root length, root/stem fresh and dry weight, Dualex values (Nitrogen balance index (NBI), flavonol, anthocyanin, and chlorophyll), leaf area, leaf temperature and color values (L*, a) *, b * C and Hue° were examined. All EB applications increased the plant height and leaf area. Also, the majority of EB isolates enhanced the root dry weight. The effect of EB applications on flavonol and chlorophyll content was not found statistically significant. However, there was a statistically significant increase in the nitrogen balance index (NBI). It was also observed that EB applications caused changes in plant color. According to the results obtained, it has been seen that it is possible to produce environmentally friendly and sustainable medicinal sage with appropriate plant-bacteria combinations.
Endophytic bacteria (EB) may contribute to plant growth and health in plants growing under stress. In this context, the study aimed to isolate endophytic bacteria from wild plants grown under saline conditions and determine plant growth-promoting (PGP) mechanisms and evaluate their effects on cucumber plant biomass. Methods and Results: Different isolates of EB were isolated from plants growing in a natural salt source area in the Lake Van basin. In order to determine the PGP properties of these isolates; ability to dissolve phosphate, ACC-D activity, siderophore production and growth at different salt (NaCl) concentrations of these isolates were investigated. Their effects on plant growth were tested on cucumber plants under climatic chamber conditions. The 62 EBs were isolated from 26 plants belonging to Amaranthaceae, Poaceae, Zygophyllaceae, Fabaceae, and Chenopodiaceae, Hypericaceae families. It was determined that 40% of these isolates had phosphatase activity and 97% had the ability to produce siderephores. Furthermore, it was determined that 92% of the isolates could grow at 0.85M, 58% at 1.28M, 8% at 1.7M and only one isolate could grow at 2.56M of NaCl concentration. Some EB isolates significantly increased the plant shoot and root fresh and dry weight. Conclusions: Plants growing under stress in nature have a very high potential in terms of hosting bacteria that can contribute to their struggle in adverse environmental conditions and support plant health and growth. Significance and Impact of the Study: Obtained PGPR isolates with certain characters able to survive under stress, it has been observed that the preference of plants growing under pressure may increase the chance of success.
Endophyte bacteria (EB), is a subgroup of plant growth-promoting bacteria (PGPB) living in plant tissues, has an important potential in controlling plant diseases. In this study, it was aimed to determine the effects of four different EB isolates on controlling the bacterial cancer and wilt disease caused by the Clavibacter michiganensis subsp.michiganensis (Cmm), and on plant growth. EB suspensions with a density of 10 8 CFU/mL were applied to tomato seedlings grown in peat/perlite mixture by drenching method twice. The suspension of Cmm at a density of 10 8 CFU/mL was applied by rubbing with piece of cotton to the third compound leaf of the
Bu çalışmada, domateste bazı hastalık ve zararlılar için geliştirilen SCAR ve CAPS markörlerinin moleküler markör destekli seleksiyon çalışmalarında geçerliliğini kontrol etmek amaçlanmıştır. Bu amaçla Domates lekeli solgunluk virüsü (TSWV), Fusarium solgunluğu (FOL), Domates yaprak kıvırcıklığı virüsü (TYLCV), kök-ur nematodu (RKN), gibi hastalık ve zararlılara dayanıklılık için geliştirilmiş olan moleküler markörler test edilmiştir. Bu markörlerden, TSWV için SCAR Scr-001 markörü, FOL için CAPS TAO1 markörü ve P743DF3-P743DR3, P743DF1-P743DR1, At2F- At2R SCAR markörleri; TYLCV için SCAR P6-25 markörü, RKN için SCAR Mi-23 ve PMİ markörleri kullanılmıştır. Bu markörlerin kontrolü, 9’u ticari 12’si yerli çeşit ve kontrol grubu olarak da Mountain Merit, NCICELBR ve NCI123S genotipleri olmak üzere 24 domates genotipinde PCR yöntemiyle yapılmıştır. TSWV için SCAR Scr-001 markörü; FOL için TAO1 CAPS markörü ve P743DF3-P743DR3, P743DF1-P743DR1, At2-F- At2-R SCAR markörleri; TYLCV için P6-25 SCAR markörü ve RKN için SCAR Mi-23 ve PMİ markörlerinden sonuç alınabilmiştir. Bu bağlamda sonuç alınan SCAR ve CAPS markörlerinin domateste moleküler yardımlı seleksiyon çalışmalarında etkin bir şekilde kullanılabileceği sonucuna varılmıştır.
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