BACKGROUND:The increase in free oxygen radicals and proinflammatory cytokines in the ischemia-reperfusion injury caused by acute mesenteric ischemia are the key responsibilities of intestinal histopathological alterations. It has been reported that Ficus carica and its various parts contain antioxidant and anti-inflammatory compounds recently. Thus, in the present study, we aimed to investigate how Ficus carica seed oil affects intestinal ischemia-reperfusion injury in a rat model. METHODS:In this study, 50 male Wistar albino rats were randomly divided into five equal groups. Negative control (NC), sham-operated (Sham), ischemia and reperfusion (IR), 3 ml/kg/day Ficus carica seed oil (FC3), 6 ml/kg/day Ficus carica seed oil (FC6). IR, FC3 and FC6 groups underwent ischemia and reperfusion procedure for 45+120 min. Only abdominal midline laparotomy was performed in the Sham group for 165 minutes.RESULTS: Tissue levels of TNFα and IL-1β, which were proinflammatory cytokines, were significantly reduced in the FC6 group than the IR group (p<0.05). In FC3 and FC6 groups, the tissue MPO and MDA enzyme levels were significantly lower than the IR group, but there was a significantly greater decrease in the FC6 group than the FC3 group (p<0.05). SOD and CAT enzymes and reduced glutathione levels of FC3 and FC6 groups were significantly lower than IR group (p<0.05); however, there was no statistically significant difference between the FC3 and FC6 groups. FC3 and FC6 groups were histopathologically graded statistically lower than the IR group, and the FC6 group showed a significant decrease than the FC3 group (p<0.05). CONCLUSION:Oral administration of fig seed oil may reverse biochemical and histopathological findings resulting from ischemia-reperfusion injury in an experimental model of acute mesenteric ischemia in rats, probably because of its antioxidant and anti-inflammatory compounds.
Amaç: Bu çalışma, böbrek dokusunda iskemi ve reperfüzyon hasarının neden olduğu oksidatif strese karşı beta glukanın olası koruyucu etkilerini araştırmayı amaçlamaktadır. Materyal ve metod: Çalışmada 300-350 gr ağırlığında 30 adet erkek Wistar albino sıçan kullanıldı (n=10). Sıçanlar rastgele Sham kontrol, iskemi reperfüzyon grubu (İR), iskemi reperfüzyon + beta glukan grubu (IR + BG) olmak üzere üç gruba ayrıldı. Sham grubuna sol nefrektomi yapıldı, sağ böbrek histopatolojik ve biyokimyasal inceleme için alındı. İR grubunda sol nefrektomi sonrası travmatik olmayan mikrovasküler klemp ile 45 dakika iskemi prosedürü uygulandı, ardından sağ böbrekte 60 dakika reperfüzyon uygulandı. IR+BG grubunda, sıçanlara 10 gün süreyle 100 mg/kg beta glukan gastrik gavaj yoluyla uygulandı. Anestezi altında sağ böbreğe 45 dakikalık iskemi sonrası 60 dakika reperfüzyon uygulandı. Bulgular: Biyokimyasal inceleme sonucunda MDA değerleri IR grubunda Sham grubuna göre anlamlı artış gösterdi (p<0,05). IR+BG grubunda IR grubuna göre anlamlı azalma oldu (p<0,05). IR grubunda doku MPO değerlerinde Sham grubuna göre anlamlı bir artış görülürken, IR+BG grubunda anlamlı bir azalma olmadı. Ayrıca doku katalaz seviyelerinde de anlamlı bir fark yoktu. Doku GSH değerleri IR grubunda Sham grubuna göre anlamlı düşüş gösterdi (p<0,05). IR+BG grubunda IR grubuna göre anlamlı artış bulundu (p<0,05). Histopatolojik incelemede IR+BG grubunda IR grubuna göre daha az hasar saptandı. Sonuç: Tüm bu veriler, beta glukanın renal iskemi reperfüzyon hasarı üzerinde antioksidan etkiye sahip olabileceğini göstermektedir.
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