Dermal wound healing involves complex histo-molecular events aimed to repair the discontinuity of the epithelium. Employing nanometric silver particles provides an efficient antimicrobial effect for several dermal infections. The aim is to elucidate imminent advantages of silver nanoparticles, such as the possibility of modulating the epithelial cell repair process. Through the nanostructural implementation of chitosan thin films supporting silver nanoparticles, it was feasible to evaluate in vivo the efficacy and evolution of dermal recuperation after surgical damage. The characterization of chitosan silver nanoparticle films was performed by UV-visible spectra and Fourier transform infrared spectroscopy, X-ray diffraction, and high-resolution electron microscopy. An important dermal healing was accomplished in animals that were treated with chitosan films supporting silver nanoparticles, as confirmed by a histopathological analysis of the skin after 12 days of treatment. The developed chitosan thin film supporting an optimized amount of silver nanoparticles could be employed to treat diseases related to wound healing.
BackgroundSilver nanoparticles (AgNPs) have attracted considerable attention due to the variety of their applications in medicine and other sciences. AgNPs have been used in vitro for treatment of various diseases, such as hepatitis B and herpes simplex infections as well as colon, cervical, and lung cancers. In this study, we assessed the effect on proliferation, adhesion, and apoptosis in breast cancer cell lines of different molecular profiles (MCF7, HCC1954, and HCC70) exposed to AgNPs (2–9 nm).MethodsBreast cancer cell lines were incubated in vitro; MTT assay was used to assess proliferation. Adhesion was determined by real-time analysis with the xCELLingence system. Propidium iodide and fluorescein isothiocyanate-Annexin V assay were used to measure apoptosis. The transcriptome was assessed by gene expression microarray and Probabilistic Graphical Model (PGM) analyses.ResultsThe results showed a decreased adhesion in breast cancer cell lines and the control exposed to AgNPs was noted in 24 hours (p≤0.05). We observed a significant reduction in the proliferation of MCF7 and HCC70, but not in HCC1954. Apoptotic activity was seen in all cell lines exposed to AgNPs, with an apoptosis percentage of more than 60% in cancer cell lines and less than 60% in the control. PGM analysis confirmed, to some extent, the effects of AgNPs primarily on adhesion by changes in the extracellular matrix.ConclusionExposure to AgNPs causes an antiproliferative, apoptotic, and anti-adhesive effect in breast cancer cell lines cultured in vitro. More research is needed to evaluate the potential use of AgNPs to treat different molecular profiles of breast cancer in humans.
An electrically tunable multimode interference (MMI) coupler=splitter is demonstrated. The device operates by modifying the phase of the multiple self-images that are formed around the midpoint of the MMI structure. This provides a simple way to fine-tune the 50:50 output power split ratio or other arbitrary ratio.
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