The automated MagNA Pure DNA extraction method for Chlamydia trachomatis was compared with the manual Cobas Amplicor protocol using 100 microL of input sample volume from 964 specimens. Agreement between protocols was 96.1%. The automated extraction method had a sensitivity of 99% and a specificity of 100%. Amplification inhibition observed after manual preparation of samples (3.8%) was not apparent following automated extraction. Using 200 microL of sample in the automated extraction process lowered the detection limit without raising the inhibition rate. Furthermore, the automated extraction method halved the hands-on time required for the procedure.
The lipophilicity of a drug is an important parameter for its eventual development by the pharmaceutical industry. It is usually measured by HPLC following partition of the compound between water and 1‐octanol. We present here an alternative, simple, sensitive and quantitative 1H nuclear magnetic resonance (NMR) method for the experimental measurement of partition coefficients of natural compounds and pharmaceutical drugs. It is based on measuring concentrations in the water phase, before and after partitioning and equilibration between water and octanol, using the ERETIC (Electronic Reference To Access In Vivo Concentration) technique. The signal to noise ratio is improved by a Water Suppression by Excitation Sculpting sequence. Quantification is based on an electronic reference signal and does not need addition of a reference compound. The log P values of 22 natural metabolites and four pharmaceutical drugs were determined and the experimental results are in excellent agreement with literature data. The experiments were run on ~2 mg material. This technique proved to be robust, reproducible and suitable for log P values between −2 and +2.
Introduction: Traditional Chinese medicine (TCM) revolves around complex mixtures bound to specific roles within the formulation, among which saponin-containing plants with alleged properties of harmonising or detoxifying other compounds present in the preparations.Objective: This article deals with the study of these interactions with, as a model, the interaction between saponins and selected active principles.
Methods:The measurement of the partition coefficient between water and octanol (logP) was used as an indicator and determined by nuclear magnetic resonance (NMR) for these active principles in the presence of saponins. For each compound, a graph was constructed showing the evolution of logP with increasing concentrations of saponins.Results: Four distinct patterns of interactions were distinguished. Pattern A showed a constant decrease of logP, pattern B showed a decrease followed by a plateau, in pattern C the logP did not vary until the critical micellar concentration (CMC) and decreased afterwards, and pattern D exhibited an increase of logP. These properties were linked to the ability of saponins to form micelles in water once the CMC is reached. The interaction of aconitine and saponins followed pattern D, thus explaining the detoxification of herbal preparations using Aconitum with licorice. The licorice facilitated the extraction of the notoriously water-insoluble artemisinin from Artemisia annua.
Conclusion:This investigation confirms that the physical properties of micelle forming saponins are intimately linked to a modification of behaviour of the other molecules in solution, as seen with the alteration of logP and the four types of interactions presented.
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