The fermentation quality of small-bale silage and haylage for feeding to horses in Sweden, and using a conventional high-density hay baler, was investigated in two experiments. Treatments studied were use of additives (inoculants containing lactic acid bacteria and a chemical additive consisting of hexamethylenetetramine, sodium nitrate, sodium benzoate and sodium propionate), the influence of dry-matter (DM) content of wilted herbage and the effect of number of stretch film layers on fermentation pattern and aerobic stability. All silages and haylages were made from predominantly Timothy swards and were well fermented as indicated by low levels of ammonia and butyric acid. Values of pH were higher and concentrations of organic acids were lower in haylages than in the silages. This was not considered to be indicative of a poor fermentation in the haylage but of a restricted fermentation due to the high DM content of the herbage. The additives enhanced aerobic storage stability because of inhibition of mould growth. The only statistically significant effect of varying the number of stretch film layers was a higher content of CO 2 inside the bales when ten layers of stretch film were applied compared with six layers.
An experiment investigating changes in fermentation variables and microbial composition during storage of small-bale silage and haylage was performed. Haylage was defined as silage with a dry-matter (DM) content exceeding 500 g kg )1 . Grass was wilted to three different DM contents, and baled into silage (350 g DM kg )1 ), haylage with a low DM content (550 g kg )1 ) and haylage with high DM content (700 g kg )1 ) in small rectangular-shaped bales (0AE80 m · 0AE48 m · 0AE36 m) that were individually wrapped. Bales were stored for short (2 months) or long (14 months) periods before opening and sampling. Silage had higher concentrations of fermentation products and a lower pH than either of the two haylage types. In general, long-term storage influenced all fermentation variables except concentrations of butyric acid and succinic acid, and yeast counts were higher after 14 months than 2 months of storage. However, silage changed in more fermentation variables during storage than the two haylage types. Although differences between short-and long-term stored silage were found, linear correlations between short-and long-term stored bales were present for all chemical variables. Pearson's correlation coefficients were highest for lactic acid, followed by pH.
Wrapped forages such as haylage and silage are increasing in popularity in equine feed rations, but knowledge of their microbial composition, especially of haylage, is scarce. Haylage may be more at risk of mould growth compared to silage, and methods for the assessment of hygienic quality of haylage are needed. Varying culturing conditions, including aerobic ⁄ anaerobic incubation, four substrates and three incubation temperatures were therefore evaluated for analysis of fungi in haylage samples from eighteen horse farms, taken on two occasions (autumn and the following spring). Average mould counts in forage samples were low, but fungi with the potential for producing mycotoxins, such as Aspergillus fumigatus and Penicillium spp., were present. The microbial composition and content of fermentation products were similar in haylage samples taken in autumn and in spring. The type of substrate used for cultivation did not influence mould or yeast counts, but not all mould species were present on all substrates. Incubation temperature influenced fungal counts and the presence of mould species. By using at least two substrates (malt extract agar and dichloran 18% glycerol agar) and two incubation temperatures (25 and 37°C), all mould species ⁄ genera that were identified in this study could be detected.
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