Shiga toxin-producing Escherichia coli (STEC) and Listeria monocytogenes are worldwide recognized zoonotic pathogens. Recent reports have emerged about the circulation of antimicrobial-resistant STEC and L. monocytogenes isolates. To assess the frequency of antimicrobial resistance and related genes in these pathogens, we studied 45 STEC and 50 L. monocytogenes isolates locally recovered from different sources. Antimicrobial susceptibility testing was performed by disk-diffusion method, and the genomic sequences of three selected STEC and from all 50 L. monocytogenes isolates were analyzed for antibiotic resistance genes. Four STEC and three L. monocytogenes isolates were phenotypically resistant to at least one of the antibiotics tested. Resistance genes aph(3 ′′)-Ib, aph(3 ′)-Ia, aph(6)-Id, bla T EM−1B , sul2, mef(A), and tet(A) were found in a human STEC ampicillin-resistant isolate. All L. monocytogenes isolates harbored fosX, lin, mdrL, lde fepA, and norB. Overall resistance in L. monocytogenes and STEC was low or middle. However, the high load of resistance genes found, even in susceptible isolates, suggests that these pathogens could contribute to the burden of antimicrobial resistance.
Comamonas kerstersii es un bacilo gramnegativo no fermentador, asociado a infecciones intraabdominales. Comunicamos un caso clínico de una bacteriemia por C. kerstersii en un joven con una apendicitis aguda. La primera identificación, mediante el sistema Vitek2 compact (BioMerieux) desde los hemocultivos, fue Comamonas testosteroni. Sin embargo, mediante MALDI-TOF se identificó como C. kerstersii. El paciente fue tratado con ampicilina/sulbactam y luego piperacilina/ tazobactam con una evolución favorable. Dentro del género Comamonas, C. testosteroni ha sido la especie más frecuentemente reportada como causa de infecciones en humanos, describiéndose solo 23 casos de C. kerstersii. Dado los problemas en la identificación fenotípica de estos microorganismos, es posible que algunos de los reportes de C. testosteroni pudieran haber correspondido a C. kerstersii. Este caso pone en evidencia el rol patógeno de C. kerstersii y la importancia de utilizar MALDI-TOF como herramienta diagnóstica en bacilos gramnegativos no fermentadores.
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