The encapsulation of enzymes within silica gels has been extensively studied during the past decade for the design of biosensors and bioreactors. Yeast spores and bacteria have also been recently immobilized within silica gels where they retain their enzymatic activity, but the problem of the long-term viability of whole cells in an inorganic matrix has never been fully addressed. It is a real challenge for the development of sol-gel processes. Generic tests have been performed to check the viability of Escherichia coli bacteria in silica gels. Surprisingly, more bacteria remain culturable in the gel than in an aqueous suspension. The metabolic activity of the bacteria towards glycolysis decreases slowly, but half of the bacteria are still viable after one month. When confined within a mineral environment, bacteria do not form colonies. The exchange of chemical signals between isolated bacteria rather than aggregates can then be studied, a point that could be very important for 'quorum sensing'.
A wide variety of biomolecules, ranging over proteins, enzymes,
antibodies and even whole cells, have been embedded within sol-gel
glasses. They retain their bioactivity and remain accessible to
external reagents by diffusion through the porous silica. Sol-gel
glasses can be cast into desired shapes and are optically
transparent, so it is possible to couple optics and bioactivity
to make photonic devices and biosensors. The high specificity and
sensitivity of enzymes and antibodies allows the detection of traces
of chemicals. Entrapped living cells can be used for the production
of metabolites, the realization of immunoassays and even for cell
transplantation.
The viability of bacteria in the presence of sol-gel reagents has been studied in order to define the best experimental conditions for the sol-gel encapsulation of E. coli. The b-galactosidase activity of these bacteria, trapped in sol-gel silica matrices, was then analyzed. Two routes, using alkoxide and aqueous precursors, have been used and compared. It appears that the aqueous route is less damaging than the alkoxide one. Moreover the aqueous silica matrix appears to slow down the lysis of cell membranes when bacteria are aged without nutrient.
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