SummaryWe measured two ectoenzymes, ecto-5'-nucleotidase (NT) and dipeptidy! peptidase IV (DP) in the peripheral blood lymphocytes of various groups of HI V-infecled patients because of the previous implied relationship of these enzymes to immune funetion.NT expressed as mean nmol/h per mg protein (±s.d.) was significantly depressed in the HlV-seropositive asymptomatic (42 ± 32; /'
Summary We describe a simple, non-radioactive, semi-automated method for measuring lymphocyte ccto-5'-nucleotidase activity in isolated cell homogenates. The method which uses inosine 5'-monophosphate(IMP)asa subslrate was optimized and requires a total of 4x 10*' lympbocytes. The reference range obtained (38 7-180 0 nmol/h per mg protein; .v= 106-6) agrees with other isotopic methods which use ''*C-IMP. The simplicity, sensitivity (lower limit 10, upper limit 400 nmol/h per mL) and precision (at 114 nmol/h per mL, interday4-2%s.d.,M = 20; intraday 5-8%, n * 10) makes this method suitable for routine eliniea! laboratory use.
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