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Background: Anaerobic digestion (AD) of protein-rich grass silage was performed in experimental two-stage twophase biogas reactor systems at low vs. increased organic loading rates (OLRs) under mesophilic (37°C) and thermophilic (55°C) temperatures. To follow the adaptive response of the biomass-attached cellulolytic/hydrolytic biofilms at increasing ammonium/ammonia contents, genome-centered metagenomics and transcriptional profiling based on metagenome assembled genomes (MAGs) were conducted.

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No abstract

The use of recently developed magnetic foam glass particles for immobilization of microbial biomass was tested. The effect of the particles was illustrated at the production of biogas from sugar beet silage as the sole substrate. Lab-scale fermentation experiments were conducted using a mesophilic completely stirred tank reactor and a magnetic separator. Microscopic analysis revealed biofilm coverage of 50-60% on the surface of the particles within 110 days. It was possible to recover 76.3% of the particles from fermentation effluent by means of a separation procedure based on magnetic forces. Comparing a particle charged reactor with a control reactor showed a small performance gain. The methane rate was increased from 1. were achieved. Based on the results, it can be concluded that the use of magnetic particles could be an attractive option for the optimization of biogas production.