The influence of salicylic acid (SA) on elicitation of defense mechanisms in Arabidopsis (Arabidopsis thaliana) seeds and seedlings was assessed by physiological measurements combined with global expression profiling (proteomics). Parallel experiments were carried out using the NahG transgenic plants expressing the bacterial gene encoding SA hydroxylase, which cannot accumulate the active form of this plant defense elicitor. SA markedly improved germination under salt stress. Proteomic analyses disclosed a specific accumulation of protein spots regulated by SA as inferred by silver-nitrate staining of two-dimensional gels, detection of carbonylated (oxidized) proteins, and neosynthesized proteins with [ 35 S]-methionine. The combined results revealed several processes potentially affected by SA. This molecule enhanced the reinduction of the late maturation program during early stages of germination, thereby allowing the germinating seeds to reinforce their capacity to mount adaptive responses in environmental water stress. Other processes affected by SA concerned the quality of protein translation, the priming of seed metabolism, the synthesis of antioxidant enzymes, and the mobilization of seed storage proteins. All the observed effects are likely to improve seed vigor. Another aspect revealed by this study concerned the oxidative stress entailed by SA in germinating seeds, as inferred from a characterization of the carbonylated (oxidized) proteome. Finally, the proteomic data revealed a close interplay between abscisic signaling and SA elicitation of seed vigor.

Abstract.A new theoretical approach to spin-isospin excitations in open-shell nuclei is presented. The developed method is based on the relativistic meson-exchange nuclear Lagrangian of Quantum Hadrodynamics and extends the response theory for superfluid nuclear systems beyond relativistic quasiparticle random phase approximation in the proton-neutron channel (pn-RQRPA). The coupling between quasiparticle degrees of freedom and collective vibrations (phonons) introduces a time-dependent effective interaction, in addition to the exchange of pion and ρ-meson taken into account without retardation. The time-dependent contributions are treated in the resonant time-blocking approximation, in analogy to previously developed relativistic quasiparticle time blocking approximation (RQTBA) in the neutral (non-isospin-flip) channel. The new method is called proton-neutron RQTBA (pn-RQTBA) and applied to Gamow-Teller resonance in a chain of neutron-rich Nickel isotopes 68−78 Ni. A strong fragmentation of the resonance along with quenching of the strength, as compared to pn-RQRPA, is obtained. Based on the calculated strength distribution, beta-decay half-lives of the considered isotopes are computed and compared to pn-RQRPA half-lives and to experimental data. It is shown that a considerable improvement of the half-life description is obtained in pn-RQTBA because of the spreading effects, which bring the lifetimes to a very good quantitative agreement with data.PACS. 21.60.Jz Nuclear Density Functional Theory and extensions (includes Hartree-Fock and randomphase approximations) -23.40.-s beta-decay; double beta-decay; electron and muon capture -21.10.Tg Lifetimes, widths

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The nuclear response theory for isospin-transfer modes in the relativistic particle-vibration coupling framework is extended to include coupling of single nucleons to isospin-flip (charge-exchange) phonons, in addition to the usual neutral vibrations. This new coupling introduces dynamical pion and rho-meson exchange, beyond the Hartree-Fock approximation, up to infinite order. We investigate the impact of this new mechanism on the Gamow-Teller response of a few doubly-magic neutron-rich nuclei, namely 48 Ca, 78 Ni, 132 Sn and 208 Pb. It is found that the coupling to isospinflip vibrations can have a non negligible impact on the strength distribution and quenching of the giant resonance, globally improving the agreement with the experimental data. The corresponding beta-decay half-lives of 78 Ni and 132 Sn are also calculated, and found to be decreased by a factor ∼ 2 by the inclusion of the new phonons.