In the cochlea of the adult Bronx waltzer (bv/bv) mouse, the majority of inner hair cells are missing or deformed. As a result, Bronx waltzer mice are severely hearing impaired or deaf. Previous studies determined that most inner hair cells in these mice are missing by the time of birth, but no studies have resolved whether the missing inner hair cells ever exist in the mutant cochlea. The present study used light and electron microscopy to locate inner hair cells in the mutant mouse before birth. Most, and possibly all, inner hair cells exist in the embryonic day (E) 17 mouse. The shapes of the cells vary from normal and elongated in the youngest animals, to round and protruding through the reticular lamina a few days later. The density of sensory cells in the inner hair cell region (inner hair cells/millimeter) decreases in the basal turn between E17 and birth, and in the apical turn between birth and the third postnatal day. The initial presence of the full complement of inner hair cells, taken together with the temporospatial pattern of degeneration, suggests that the cause of inner hair cell death in the Bronx waltzer mouse is related to a differentiation event subsequent to cell birth.
In the cochlea of the adult Bronx waltzer (bv/bv) mouse, the majority of inner hair cells are missing or deformed. As a result, Bronx waltzer mice are severely hearing impaired or deaf. Previous studies determined that most inner hair cells in these mice are missing by the time of birth, but no studies have resolved whether the missing inner hair cells ever exist in the mutant cochlea. The present study used light and electron microscopy to locate inner hair cells in the mutant mouse before birth. Most, and possibly all, inner hair cells exist in the embryonic day (E) 17 mouse. The shapes of the cells vary from normal and elongated in the youngest animals, to round and protruding through the reticular lamina a few days later. The density of sensory cells in the inner hair cell region (inner hair cells/millimeter) decreases in the basal turn between E17 and birth, and in the apical turn between birth and the third postnatal day. The initial presence of the full complement of inner hair cells, taken together with the temporospatial pattern of degeneration, suggests that the cause of inner hair cell death in the Bronx waltzer mouse is related to a differentiation event subsequent to cell birth.
Serum from a patient suspected of having AIDS showed positive ELISA tests but the diagnostic p 41 band was absent and the p 24 band was barely discernible on a Western blot. Before another Western blot was performed lentivirions were detected between dendritic reticulum cells in a lymph node biopsy. It is suggested that samples of biopsies of lymph nodes from patients with or at risk for AIDS should be embedded in resin for future ultrastructural study if indicated.
Virions resembling the AIDS-associated retroviruses HTLV-III and LAV were identified between pseudopods of dendritic reticulum cells in lymph nodes from 3 patients with prodromal AIDS. The virions are morphologically distinguishable from HTLV-I and HTLV-II and also from D-type retroviruses that are associated with the simian acquired immune deficiency syndrome. Budding forms resembling incomplete nucleoids of maturing D-type retrovirus were found in neutrophils in a lymph node from another patient with prodromal AIDS. The significance of these structures is unknown.
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