The short-term dynamics and distribution of airborne biological and total particles have been assessed in a large university hallway by particle counting using laser particle counters and impaction air samplers. Particle numbers of four different size ranges were determined every 2 min over several hours. Bioaerosols (culturable bacteria and fungi determined as colony-forming units) were directly collected every 5 min on Petri dishes containing the appropriate growth medium. Results clearly show distinct short-term dynamics of particulate aerosols, of both biological and non-biological origin. These reproducible periodic patterns are closely related to periods when lectures are held in lecture rooms and the intermissions in between when students are present in the hallway. Peaks of airborne culturable bacteria were observed with a periodicity of 1 h. Bioaerosol concentrations follow synchronously the variation in the total number of particles. These highly reproducible temporal dynamics should be considered when monitoring indoor environments to determine air quality.
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