A single dose of 0.25 ng of tetanus toxin (TeTx), equivalent to -5 minimal lethal doses, injected intracerebrally to I -day-old rats, caused translocation, i.e., activation, of Ca'+-phosphatidylsenne-dependent protein kinase C (PKC) from the cytosolic to the membrane compartment within 1 h. Six hours after treatment with the toxin, a 40-50% reduction in the total brain PKC (cytosolic plus membrane) activity was noticed. G T l b (2 pg per brain) ganglioside, a putative receptor for TeTx, completely prevented enzyme translocation when injected intracerebrally 30 min before toxin administration and abolished down-regulation after 6 h from the time of toxin injection. GM1 (2 pg per brain), a ganglioside of lesser affinity for TeTx, produced by itself a 20-30% reduction of the total PKC activity and did not reverse TeTx-induced PKC down-regulation after 6 h.
12-O-Tetradecanoylphorbol
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