La satisfacción de consumidores y usuarios con los productos y servicios es un tema que en los últimos años ha ido adquiriendo cada vez más importancia hasta alcanzar también el campo de la psicoterapia. En este trabajo se presenta la adaptación al español de la Escala de Satisfacción con el Tratamiento Recibido (CRES-4). Tiene cuatro ítems y fue creada para evaluar el grado de satisfacción del cliente con la terapia recibida, el grado en que considera que su problema principal se ha resuelto y el cambio percibido en su estado emocional del pretratamiento al postratamiento. La puntuación global pretende reflejar la eficacia del tratamiento según el paciente (Nielsen et al., 2004). Los estudios realizados hasta la fecha con este instrumento sugieren que la CRES-4 puede ser un buen instrumento complementario, especialmente si se quiere evaluar la satisfacción con la psicoterapia.
The methyl erythritol phosphate (MEP) pathway of isoprenoid biosynthesis is essential for malaria parasites and also for several human pathogenic bacteria, thus representing an interesting target for future antimalarials and antibiotics and for diagnostic strategies. We have developed a DNA aptamer (D10) against Plasmodium falciparum 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), the second enzyme of this metabolic route. D10 binds in vitro to recombinant DXR from P. falciparum and Escherichia coli, showing at 10 µM a ca. 50% inhibition of the bacterial enzyme. In silico docking analysis indicates that D10 associates with DXR in solvent-exposed regions outside the active center pocket. According to fluorescence confocal microscopy data, this aptamer specifically targets in P. falciparum in vitro cultures the apicoplast organelle where the MEP pathway is localized and is, therefore, a highly specific marker of red blood cells parasitized by Plasmodium vs. naïve erythrocytes. D10 is also selective for the detection of MEP+ bacteria (e.g., E. coli and Pseudomonas aeruginosa) vs. those lacking DXR (e.g., Enterococcus faecalis). Based on these results, we discuss the potential of DNA aptamers in the development of ligands that can outcompete the performance of the well-established antibody technology for future therapeutic and diagnostic approaches.
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