During exocytosis, the fusion pore expands to allow release of neurotransmitters and hormones to the extracellular space. To understand the process of synaptic transmission, it is of outstanding importance to know the properties of the fusion pore and how these properties affect the release process. Many proteins have been implicated in vesicle fusion; however, there is little evidence for proteins involved in fusion pore expansion. Myosin II has been shown to participate in the transport of vesicles and, surprisingly, in the final phases of exocytosis, affecting the kinetics of catecholamine release in adrenal chromaffin cells as measured by amperometry. Here, we have studied single vesicle exocytosis in chromaffin cells overexpressing an unphosphorylatable form (T18AS19A RLC-GFP) of myosin II that produces an inactive protein by patch amperometry. This method allows direct determination of fusion pore expansion by measuring its conductance, whereas the release of catecholamines is recorded simultaneously by amperometry. Here we demonstrated that the fusion pore is of critical importance to control the release of catecholamines during single vesicle secretion in chromaffin cells. We proved that myosin II acts as a molecular motor on the fusion pore expansion by hindering its dilation when it lacks the phosphorylation sites.Exocytosis is a fundamental cellular mechanism used by neurons and hormone-secreting cells to interact with each other and to influence their environment through the release of neurotransmitters and hormones. These chemical signals are disposed to the extracellular medium in the form of quanta, as vesicles containing transmitter fuse with the plasma membrane and release their cargo. Release occurs through the exocytotic fusion pore, which is the water channel connecting the vesicle interior to the extracellular space.The dynamics of the fusion pore have been mainly investigated at the level of single cells by two techniques: patch clamp measurements of the electrical capacitance of the cell membrane (1-3) and the amperometric detection of neurotransmitter with carbon fibers (4, 5). Whereas patch clamp detects changes of cell membrane area and conductance caused by vesicular fusion, the electrochemical method analyzes quantitatively the release of catecholamines from each exocytotic event.

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Designing, financing, and operating successful solar heating, concentrating solar power, and photovoltaic systems requires reliable information about the solar resource available and its variability over time. In the past, seasonal and daily variability has been studied and understood; however, with new solar technologies becoming more important in energy supply grids, small time-scale effects are critical to successful deployment of these important low carbon technologies. A vital part of the bankability of solar projects is to understand the variability of the solar resource so that supply and storage technologies can be optimized. This handbook is the result of 10 years of international collaboration carried out by experts from the International Energy Agency's (IEA's) Solar Heating and Cooling (SHC), Solar PACES, and Photovoltaic Power Systems Technology Collaboration Programmes. Under IEA SHC Task 46: Solar Resource Assessment and Forecasting, experts from 11 countries produced information products and best practices on solar energy resources that will greatly benefit project developers and system operators as well as assist policymakers in advancing renewable energy programs worldwide.Meteorologists, mathematicians, solar technology specialists, and other key solar resource experts from around the world joined forces to further our understanding of the sun's temporal and spatial variability through benchmarking satellite-derived solar resource data and solar forecasts, developing best practices for measuring the solar resource, and conducting research to improve satellite-based algorithms. The results of IEA SHC Task 46 are useful to a wide range of users of solar heating and cooling, photovoltaics, and concentrating solar power systems and of building developers and owners as well as anyone else who needs to understand and predict sunlight for agricultural or other purposes.The earlier edition of the handbook, which was published in 2015, is used worldwide as a reference for each stage of a solar energy project. Since that time, there has been substantial growth in the interest in high-quality "bankable" solar resource data. This revision adds significant new methods so it will be even more useful. This publication is a summary that details the fundamentals of solar resources as well as captures the state of the art. For those wanting more depth, it also provides the references where more detailed information can be found. I would like to acknowledge the leadership of the National Renewable Energy Laboratory and express appreciation to the U.S. Department of Energy for producing the handbook and incorporating results from IEA SHC Task 46.

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