Purpose: The purpose of the study is to investigate the cytotoxic effect of sevoflurane (SF) and povidone‐iodine (PI) on cultured rabbit corneal epithelial cells in vitro. Methods: We evaluated the cytotoxic effect of a 5 min exposition of SF, PI and balanced saline solution (BSS) using an in vitro rabbit corneal cell culture model (Short‐Time Exposure Test Method). In order to quantify cytotoxicity MTT assay was employed. Repeated exposition of SF was evaluated as well as cell viability after different recovery times (30 min, 60 min and 1 h). Statistical results were obtained with Kruskal‐Wallis test and Conover post‐hoc test. Results: The median of cell viability of SF was 85.29%, PI 0.52% and BSS 97.29%, with statistically significant differences between them (p = 0.005). After a 5 minute recovery, the median of cell viability for SF was 87.77%, 74.48% after 30 minutes and 76.66% after 60 minutes. After a double exposition it was 70.59%, with no statistically significant differences between single and double exposition (p = 0.18). Conclusions: No corneal toxicity of SF was observed, in contrast with PI. Cellular damage did not change with longer recovery time or double exposition to SF. References Sevoflurane (SF) has been proved recently to have analgesic/antimicrobial effects. (1, 2) We believe of interest to study the effect of SF in the ocular surface. As there is no literature about this topic, in vitro studies are required before in vivo tests. 1. Chu CC, Wu SZ, Su WL, Shieh JP, Kao CH, Ho ST, et al. Subcutaneous injection of inhaled anaesthetics produces cutaneous analgesia. Can J Anesth Can Anesth. 2008;55(5):290–4. 2. Manuel Gerónimo Pardo, María Martínez Serrano, Ángel Martínez Monsalve, Juan Luis Rueda Martínez. Usos Alternativos del Sevoflurano: Efecto Analgésico Tópico. Rev Electrónica AnestesiaR [Internet]. 2012 [cited 2019 Apr 15];4(5). Available from: http://revistaanestesiar.org/index.php/rear/article/view/373
Purpose: To determine sevoflurane (SF) ocular toxicity in an animal model and compare the results with the changes induced by common ocular substances: Povidone Iodine 5% (PI) and salt solution (SS). Methods: Twelve rats (24 eyes) and 3 rabbits (6 eyes) where included. Eyes were randomized, receiving a single 0,1 ml dose of SF, PI or SS. After instillation, eyes were evaluated using Draize test at different time frames. A histopathological (HP) study was also performed. Results: Keratitis was the only finding identified in the animals. In rats, it was observed in 16/24 eyes (8 in SS, 4 in SF and 4 in PI group). Only the keratitis observed after SS and PI instillation showed statistically significant differences, with a higher incidence in PI group (p = 0.012). Similar results were observed in rabbits: keratitis was present in 5/6 eyes (3 in SF and 2 in BSS group), but no significant differences were observed in any group at any time of study. The risk ratio (=1) did not show a higher risk of keratitis with any substance. In HP study, only mild inflammatory changes were observed. Conclusions: Topical administration of SF has shown to be safe for the eye surface of animal model. References Recent findings suggest sevoflurane role as an analgesic, antiseptic and wound‐healing agent. Nevertheless, previous to study these effects on the eye, it is necessary to perform and toxicological test. This is the first study performed in vivo to evaluate sevoflurane ocular toxicity.
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