Development rates, nitrogen-and carbon-specific growth rates, size, and condition were determined for the copepod Calanus finmarchicus reared at 3 temperatures (4, 8, and 12°C) at non-limiting food concentrations and 2 limiting food concentrations at 8°C in the laboratory. Development rates were equiproportional, but not isochronal. Naupliar stage durations were similar, except for non-feeding stages, which were of short duration, and the first feeding stage, which was prolonged, while copepodite stage durations increased with increasing stage of development. Under limiting food concentrations at 8°C, development rates were prolonged but similar relative patterns in stage durations were observed. Body size (length and weight) was inversely related to temperature and positively related to food concentration. Condition measurements were not affected by temperature, but were positively related to food concentration. Growth rates increased with increasing temperature and increased asymptotically with increasing food concentration. At high food concentrations, growth rates of naupliar stages were high (except for individuals molting from the final naupliar stage to the first copepodite stage, in which growth rates were depressed), while growth of copepodites decreased with increasing stage of development. Neither nitrogen nor carbon growth rates, the former a proxy for structural growth, were exponential over the entire life cycle, but rather sigmoidal. Carbon-specific growth rates were greater than nitrogen-specific growth rates, and this difference increased with increasing stage of development, reflecting an augmentation in lipid deposition in the older stages. However, nitrogen and carbon growth rates were more similar under foodlimited conditions. Based on this study, we recommend that secondary production rates of Calanus finmarchicus and possibly other lipid-storing copepods not be estimated from egg production measurements alone, as has been suggested for other species of copepods, because growth, including structural growth, is not equivalent for all stages.
We examined the effects of food concentration and temperature on nucleic acids and protein content of Calanus finmarchicus in order to evaluate the use of RNA as a growth rate index for this species. We measured RNA, DNA, and protein content of copepods reared from egg to adult stage in 5 combinations of food and temperature conditions (25 to 500 µg C l -1 , 4 to 12°C). At 8°C, DNA, RNA and protein content and RNA:DNA differed among food treatments during Stages N6 through to adult female. Protein:DNA ratios and RNA:protein ratios were significantly different among food levels for only 3 of the 8 stages examined. At excess food, DNA, RNA, and protein content and RNA:DNA ratios were inversely related to temperature for most stages from C1 onward, but the effect of temperature was relatively small over the range of temperatures investigated. The RNA:DNA and protein:DNA ratios increased with developmental stage whereas the RNA:protein ratio and growth rates (measured in terms of protein, nitrogen, DNA, and carbon content) declined with increasing stage. Although the relationship of RNA:DNA to growth rates was stage-specific, the two were related when standardized for temperature and developmental stage. RNA:protein ratios were directly related to growth rates regardless of stage, and the slope of the relationship increased with increasing temperature in a nonlinear fashion. Our results emphasize the importance of temperature and developmental stage for the relationship of growth rates to RNA concentration and RNA:DNA ratios. We propose 2 ways to estimate in situ growth rates of C. finmarchicus from RNA:DNA or RNA:protein ratios and environmental temperature.
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