No abstract

separate mixtures of inorganic, ester, and nucleotide phosphates and to recover each of these in substance. The application of this information will, of necessity, be an individual one, and it seems not worth while to recommend a specific procedure of limited application. It must be kept in mind that differences in the degree of adsorption and desorption of esters and nucleotides as observed here will be encountered, and control experiments will be required (7).The growth of artificial insemination of cattle during the past 10 years has emphasized the need for adequate methods of predicting the fertility of semen samples before they are used. Laboratory studies and field operations have relied heavily on visual estimates of sperm numbers and sperm motility. These estimates have been continuously subject to the unavoidable human errors and biases encountered in collecting quantitative data with the microscope. A number of objective methods have been proposed for evaluating sperm motility (1-3). These methods have made use of the relationships among sperm numbers, area observed, distances traveled, and the time intervals between or during the observations. The objections to these methods have included such items as the need for standardization of the sperm numbers, the laborious and time-consuming techniques and calculations, the human bias, and the repeated high costs of the determinations.The ideal method of evaluating sperm separate mixtures of inorganic, ester, and nucleotide phosphates and to recover each of these in substance. The application of this information will, of necessity, be an individual one, and it seems not worth while to recommend a specific procedure of limited application. It must be kept in mind that differences in the degree of adsorption and desorption of esters and nucleotides as observed here will be encountered, and control experiments will be required (7).