2018) Characterization and phylogenetic analysis of the complete mitochondrial genome from Rock Scallop (Crassadomagigantea) using next-generation sequencing,
ABSTRACTIn this study, the complete 18,495 bp mitochondrial genome was determined from Rock scallop (Crassadoma gigantea) using next-generation sequencing technology. The complete mitochondrial genome contained 12 protein-coding genes (PCGs), 2 ribosomal RNA genes, 23 transfer RNA genes, without ATP8 and D-loop, which was similar with most mitochondrial genomes of marine bivalve molluscs. Gene annotations, including gene order, genetic code, start and stop codons and codons bias, were identified. Phylogenetic tree was constructed using Neighbor-Joining (NJ) method based on the PCGs showed the present species clustered within the Pteriomorphia clade. This work should be of importance not only for the better understanding of the relationships within Pectinidae, but also for the development of useful genetic markers in Rock scallop aquaculture and restoration efforts.
ARTICLE HISTORY
The effect of the bacterial strain CI4 on the germination of spores from the green alga Ulva pertusa was assayed and it was found that the bacterial biofilm and cell-free supernatant strongly inhibited spore germination. In attempts to define the chemical nature of the antifouling substance in the supernatant of CI4, the culture supernatants were tested for activity after heat treatment, enzymatic treatments, size fractionation, and separation into aqueous and organic fractions. Results suggest that this bacterium produces an extracellular component with specific activity toward algal spores that was heat-sensitive and between 3 and 10 kDa in molecular size. The exposure of the organic phase fraction to spores showed inhibitive effect on spore germination. Pronase and carboxypeptidase y did not significantly affect the activity of inhibitory component, suggesting that the component was not a protein or a peptide. The bacterium CI4 was identified as Pseudoalteromonas haloplanktis based on the phenotypic characters and 16S rRNA gene analysis.
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