In the last decade, our understanding of rice domestication has improved by new archaeological findings using advanced analytical techniques such as morphological and morphometric analyses on rice grains, spikelet bases and phytoliths, and ancient DNA analysis on rice remains. Previous studies have considered the size of rice bulliform phytoliths as a proxy for tracking the domestication process. These phytoliths are often abundant and well preserved in sediments, and their shape is under the control of numerous genes, which may shift toward larger sizes by genetic mutation in domestication. Therefore, it has been assumed that the bulliforms of domesticated rice are usually larger than those of wild ones; however, morphometric data supporting this assumption are lacking in the literature, thereby requiring additional evidence to test its veracity. In this study, the vertical and horizonal lengths of bulliform phytoliths were measured in four rice species (domesticated Oryza sativa and wild Oryza rufipogon, Oryza officinalis, and Oryza meyeriana) from different regions of southern China. We found that the bulliform morphometric data of wild and domesticated rice overlapped and that there was no statistically significant difference between them. Therefore, bulliform size could not be used as a diagnostic indicator to distinguish domesticated rice from wild species and is a supporting rather than conclusive proxy for determining the domesticated status of rice in archaeological research. We further found that larger rice bulliform sizes likely occurred at the locations with higher temperature, precipitation, and water levels, indicating hydrothermal environment is an alternative factor influencing the size of rice bulliform phytoliths. For further archaeological use of an increasing size trend of bulliform phytoliths to reveal the process of rice domestication, we present some suggestions for controlling the influence of hydrothermal factors. Even so, the combination of bulliform phytolith size with other established criteria is strongly suggested to provide precise identification of wild and domesticated rice in future research.

Highlights d We investigate the importance of culture in achieving the Sustainable Development Goals (SDGs) d Culture mediates the attainment of 17 SDGs, represented by 79% of the SDG targets d Cultural values explain as much as 26% of the variations in the achievement of the SDGs d Sustainable policies can be tailored to, but not captive to, cultural context

Mixed millet and rice agriculture is a unique agricultural style of China, and is distributed in a broad band between the Yangtze and Yellow River basin. However, the development of this style during the Neolithic-Bronze Age has not been comprehensively clarified, owing to limited archaeobotanical data and imprecise chronology for most of the regions. Here, the Central Plain, a location where mixed agriculture may have first appeared, was selected as the area for research. Phytolith and macrofossil analyses from the Zhuzhai site, together with the accelerator mass spectrometer (AMS) radiocarbon dating of samples, reveal information about the temporal changes of mixed agriculture as well as the domestication and cultivation of crops in this region. The results indicate that mixed millet and rice agriculture formed in the Central Plain about 8000 years ago. Common millet was the principal crop in the Peiligang and Yangshao periods, with the domestication process beginning in the Peiligang period and continuing up to the Shang period, at which time it was replaced by foxtail millet. Foxtail millet may have gone through a significant degree of domestication by ca. 6000 cal. BC, but its domestication process was still unclear. Rice had appeared since the Peiligang period, but its proportions were always low. Rice assigned to the Peiligang and Yangshao Cultures was the domesticated japonica, and its cultivation was always performed in dry field systems through the Neolithic-Bronze time. Within the subsistence economy, mixed agriculture was a minor component during the Peiligang period, but has been dominant since the Yangshao period.