Açai fruit has been studied for its antioxidant properties, with positive feedback against many diseases, including cancer. Although açai seeds are not edible, their composition has been studied in order to find new applications and reduce garbage generation. This study aimed to evaluate the cytotoxic effects and impacts on the cell cycle and apoptosis of açai seed extract (ASE) on human lung carcinoma cell line (A549). Antioxidant activity of açai seed extract (ASE) was measured by DPPH assay, Trolox Equivalent Antioxidant Capacity (ABTS/TEAC), Ferric Reducing Ability (FRAP) and Oxygen radical absorbance capacity (ORAC) assays. Human lung carcinoma cell viability (A549) was monitored by MTT assay method and the effects on cell cycle and apoptosis were measured by flow cytometry. The results indicate high antioxidant activity in ASE and high values of total phenolic compounds (37.08 ± 8.56 g gallic acid/100 g). The MTT assay showed a maximum decrease (72.07%) in the viability of A549 cells after 48 h treatment with ASE (200 µg/mL). Flow cytometer analysis revealed that ASE increased the percentage of cells in G0/G1 phase and promoted a high increase of apoptotic cells when compared to the untreated cells. The present study suggests that ASE has a high antioxidant capacity and may have a protective effect against lung cancer.
INTRODUÇÃO: O estado nutricional e a avaliação do gasto metabólico têm impacto significativo na evolução clínica dos pacientes. OBJETIVO: Identificar quais métodos de avaliação do estado nutricional e cálculo da necessidade energética têm sido eleitos na prática clínica de nutricionistas, conhecendo os motivos que influenciaram suas escolhas. METODOLOGIA: Aplicou-se um questionário semi-estruturado, com perguntas quanti e qualitativas, por meio do autopreenchimento. RESULTADOS: A maioria dos entrevistados era do gênero feminino, formados em instituições públicas, com formação complementar. O principal local de atuação foi a internação e o único equipamento comum a todos foi a balança. Os métodos escolhidos por 100% dos profissionais foram o IMC e o método Kcal/Kg de peso. CONCLUSÃO: Apesar do conhecimento teórico-prático de diferentes métodos de avaliação nutricional e cálculo da necessidade energética houve preferência pelos de maior facilidade e praticidade, havendo pouco reconhecimento de suas limitações. Entre os motivos para tais escolhas destacam-se a falta de equipamentos e o número de profissionais por enfermaria.
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