Using microalgae for animal nutrition provides an economically viable route for microalgae-based technological innovation, especially in combination with CO 2 fixation given current global warming. However, this technology still lacks sufficient evaluation for screening microalgae for specific animals; meanwhile, current studies show some prejudice regarding 'essential' or 'non-essential' ingredients. The results show that Dunaliella salina and Nannochloropsis salina were able to accumulate high protein (30%-57%) and lipid (20%-46%) content without affecting the performance of CO 2 fixation, which reached 0.28 and 0.23 g L −1 day −1 respectively. Both species exhibited high quality of lipids and proteins for Penaeus monodon based on the profiling. The essential fatty acid indexes (EFAI) for N. salina and D. salina were 3.81 and 9.02 respectively. Butyric acid was found to be present in both D. salina (12.03%) and N. salina (4.87%) based on the total fatty acids (FAs). The essential amino acid indexes (EAAI) for D. salina and N. salina were 2.23 and 1.29 respectively. Arginine was the most abundant essential amino acid (EAA) in both D. salina (10.83%) and N. salina (13.35%) on the basis of total amino acids (AAs). This study comprehensively compares the nutritional quality of the two commercial marine microalgae of D. salina and N. salinawith the potential to be used as sustainable sources of lipids and proteins to reduce or even replace the traditional fish oil and fish meal in aquafeeds. K E Y W O R D Samino acid, aquafeed, fatty acid, lipid, microalgae, protein | 947 CHEN Et al.

High-vigor soybean seeds can result in the rapid and uniform emergence of seeds under a wide range of field conditions. We aimed to explore the quantitative trait loci of seed vigor traits and superior allelic and carrier materials from the soybean in northeast China. A total of 257 improved lines and 104 soybean landraces were used to evaluate seed vigor, using normal seedling fresh weight, normal seedling length, main root length, hypocotyl length, and germination percentage, in 2018 and 2019. In this study, the different degrees of LD was detected not only among syntenic markers but also among nonsyntenic ones, suggesting that there had been historical recombination among linkage groups. A total of 19 and 10 simple sequence repeat (SSR) markers associated with seed vigor were detected in improved lines and soybean landraces in 2018 and 2019, respectively. Our results also showed five SSR markers co-associated with two or three vigor traits. In addition, 41 superior alleles and their carrier materials were mined. Based on the elite alleles detected, the best cross combinations for improving seed vigor traits were proposed. The results demonstrate that association mapping is a valuable foundation for molecular breeding for soybean seed vigor.