SummaryThis study was carried out in two experiments. Experiment 1 was conducted with six ruminally-cannulated one-year old Kangal Akkaraman male lambs, using a crossover design with 2 periods to determine the effects of live yeast culture supplementation on rumen fermentation. Yearlings were either supplemented with 4 g/d of Saccharomyces cerevisiae (BeneSacc, 4x10 9 CFU/g) or not supplemented (control). Animals were penned individually, and were fed a diet composed of 25% forage and 75% concentrate for sixteen days. Rumen fluid was sampled on day 16, 0 h (before feeding), 3 h and 6 h after feeding. Rumen pH, the numbers of protozoa, ammonia-N and volatile fatty acids (VFA) were determined in samples. Molar proportions of acetate were decreased, and propionate was increased with supplementation of live yeast culture. No differences were observed for ruminal pH, protozoa population, total VFA and ammonia-N concentrations, between treatments. In Experiment 2, in situ nutrient degradability of barley grain, corn dried distillers grains with solubles (DDGS) and wheat straw were determined. Live yeast culture supplementation did not affect dry matter (DM) degradability of barley, DDGS or straw, crude protein (CP) degradability of DDGS, nor neutral detergent fiber (NDF) degradability of straw. But, it reduced the potential degradability of DDGS in rumen.. Keywords: Yeast, Yearling Lamb, Rumen fermentation, Degradability Canlı Maya Kültürünün (Saccharomyces cerevisiae) Toklularda Rumen Fermentasyonu ve Besin Madde Yıkılabilirliği Üzerine Etkileri ÖzetBu çalışma 2 deneme şeklinde yürütüldü. Deneme 1, canlı maya kültürü ilavesinin rumen fermentasyonu üzerine etkilerini tespit etmek için, rumen kanülü yerleştirilmiş 6 baş bir yaşlı Kangal Akkaraman erkek toklu ile çapraz deney düzenine göre iki dönem halinde yürütüldü. Grubun biri kontrol olarak tutulurken, diğerinin yemlerine günlük 4 g Saccharomyces cerevisiae içeren canlı maya (BeneSacc, KOB=4x10 9 ) kültürü ilave edildi. Hayvanlar bireysel bölmelerde tutuldu ve %25'i yonca, %75'i kesif yemden oluşan rasyonlarla 16 gün süreyle beslendi. 16. günde hayvanlardan yemleme öncesi, yemlemeden 3 ve 6 saat sonra rumen sıvısı örnekleri alındı. Örneklerde pH, protozoon sayısı, amonyak N'u ve uçucu yağ asitleri (UYA) belirlendi. Canlı maya kültürünün ilavesi ile asetik asit oranı azaldı, propiyonik asit oranı arttı. Ruminal pH, protozoon konsantrasyonu, toplam UYA'leri ve amonyak N'u, bakımından muameleler arasında farklılık gözlenmedi. İkinci denemede; arpa, kurutulmuş mısır damıtma ürünü (KMDÜ) ve samanın in situ besin madde yıkılabilirlikleri tespit edildi. Canlı maya kültürü ilavesi arpa, KMDÜ, samanın KM yıkılabilirliğini, KMDÜ'nün ham protein, samanın NDF yıkılabilirliğini etkilemedi. Fakat KMDÜ'nün rumende potansiyel yıkılabilirliğini düşürdü.

This study was aimed at determining the effects of various levels of dietary olive cake meal (OCM) on certain blood parameters and breast muscle fatty acid and cholesterol levels. For this purpose, 400 day-old Japanese quail (Coturnix coturnix japonica) chicks of both sexes were randomly assigned to four trial groups, each including five replicates of 20 animals. The quail were fed on a maize and soybean meal-based basal diet supplemented with 0%, 2.5%, 5% and 7.5% of OCM for six weeks. Dietary supplementation with 5% and 7.5% of OCM decreased total cholesterol and low-density lipoprotein cholesterol levels in the blood serum, and increased cholesterol levels in the breast muscle. Dietary supplementation with various levels of OCM decreased saturated fatty acid and polyunsaturated fatty acid levels, and increased monounsaturated fatty acid and total unsaturated fatty acid levels in the breast muscle. As a result, it was concluded that, olive cake meal, which contains a high level of olive oil, could be incorporated in poultry feed at a level of 5%, owing to its beneficial effects of decreasing serum LDL cholesterol, which plays a major role in the aetiology of cardiovascular diseases, and in reducing saturated fatty acid levels in breast muscle.

This study was aimed at investigating the effects of dietary supplementation with different levels of olive cake meal (0%, 2.5%, 5% and 7.5%) on the fattening performance and slaughter and carcass traits of quails. For this purpose, 400 one-day-old Japanese quail chicks were randomly assigned to 4 groups, each with 5 replicates. The quails were fed on either a basal diet alone (Control Group) or a basal diet supplemented with olive cake meal at rates of 2.5% (Group I), 5% (Group II) and 7.5% (Group III) for a period of 42 days. At the end of the fattening period, dietary olive cake meal was observed not to have caused any adverse effect on body weight, feed intake and feed conversion rate. Eviscerated hot carcass weight, eviscerated cold carcass weight, wing weight, breast weight, thigh weight and edible visceral organ weights were determined to have improved in the treatment groups that received dietary olive cake meal, when compared to the control group. In conclusion, dietary supplementation with olive cake meal, a waste product of the olive oil industry, at a level of 5%, was observed to produce the closest and most similar results to those of feeding on the basal diet. Thus, it is suggested that olive cake meal could be used as an alternative feedstuff for poultry. Furthermore, the use of olive cake meal as an alternative feedstuff would contribute to reducing both feed costs and environmental pollution.

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In this study, the biochemical and histopathological changes in the lungs due to lower extremity ischemiareperfusion injury and protective effect of boric acid to prevent them were investigated. The rats were divided into three groups as Group 1 (Sham group, n=9), Group 2 (Ischemia-reperfusion group, I/R, n=9) and Group 3 (Ischemiareperfusion, (I/R) + boric acid (BA), n=9). Anaesthesia was applied to the first group of rats without any ischemiareperfusion process. Following a two-hour ischemia, two hours of reperfusion, with the help of left lower extremities tourniquet, were applied to the second group. To the third group, 200 mg/kg (i.p.) boric acid application was performed 10 minutes before the ischemia was initiated and two hours of ischemia and two hours of reperfusion application were carried out. Some antioxidant enzymes in lung tissue (SOD, GSH-Px, CAT) were analyzed. In addition the lung tissue was evaluated histopathologically. Boric acid application was found to significantly enhance the positive effect of antioxidant enzymes. Normal histological structure was better preserved, some of macrophages were non-activated, and a remarkable reduction of neutrophil infiltration was seen after treatment in boric acid given group.