Deep learning is emerging as a powerful approach for bioimage analysis, but its wider use is limited by the scarcity of annotated data for training. We present ELEPHANT, an interactive platform for cell tracking in 4D that seamlessly integrates annotation, deep learning, and proofreading. ELEPHANT's user interface supports cycles of incremental learning starting from sparse annotations, yielding accurate, user-validated cell lineages with a modest investment in time and effort.
Deep learning is emerging as a powerful approach for bioimage analysis. Its use in cell tracking is limited by the scarcity of annotated data for the training of deep-learning models. Moreover, annotation, training, prediction, and proofreading currently lack a unified user interface. We present ELEPHANT, an interactive platform for 3D cell tracking that addresses these challenges by taking an incremental approach to deep learning. ELEPHANT provides an interface that seamlessly integrates cell track annotation, deep learning, prediction, and proofreading. This enables users to implement cycles of incremental learning starting from a few annotated nuclei. Successive prediction-validation cycles enrich the training data, leading to rapid improvements in tracking performance. We test the software’s performance against state-of-the-art methods and track lineages spanning the entire course of leg regeneration in a crustacean over 1 week (504 timepoints). ELEPHANT yields accurate, fully-validated cell lineages with a modest investment in time and effort.
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