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1 The present study investigated the binding characteristics of various ligands to cannabinoid CB 1 receptors in human neocortex and amygdala. In addition, the functionality of CB 1 receptors in the human neocortex was assessed by examining the effects of CB 1 receptor ligands on evoked

SummaryThe disruption of Cundidu utilis cells in suspensions subjected to different types of stresses was investigated. Stresses caused by impingement of a high velocity jet of suspended cells against a stationary surface were found to be significantly more effective for disruption than either shear or normal stresses. The fraction of cells disrupted by impingement is a first order function of the number of passes through the disruptor and, over a prescribed range of operating pressures, is a power function of pressure. These results indicate that impingement is the predominant mechanism causing cell disruption in high pressure flow devices such as Manton-Gaulin homogenizers. The impingement results suggest that cells grown in cyclic batch culture are easier to disrupt than cells grown at a lower specific growth rate in continuous culture. In addition to determining the fraction of cells disrupted, the release of invertase activity was determined for the impingement experiments. The fraction of total invertase activity released was found to be somewhat greater than the fraction of cells disrupted.