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In the present study, synthesis and characterisation of titanium dioxide (TiO2) nanoparticles concurrently doped with fluorine (F) and manganese (Mn) are studied for the improved photocatalytic degradation and bacterial inhibition. The absorption spectrum observed at 400 nm is blue shifted to 398 nm in co-doped TiO2, which confirmed the increase in incorporation of F and Mn. Transmission electron microscopic images and XRD results confirm that the size of the TiO2 nanoparticles decreased with increasing concentration of co-dopants. Co-doping of TiO2 with F and Mn is confirmed by FTIR, XRD, EDAX and XPS studies. The TiO2 nanoparticles prepared at higher concentration of dopants show the existence of nanorods. It is obvious that the doping of TiO2 with Mn and F can degrade textile wastewater and methylene blue with 120 min of visible light irradiation. The antimicrobial activities of the prepared samples increased with increasing doping level of F and Mn against Staphylococus aureaus and Escherichia coli.