The present communication concerns the investigations of the effect of Juvenile Hormone (J.H.) and its analogs (J.H.A.), such as Altosid (R) and Altozar(R), used as insecticides, on the biosynthesis of proteins and RNA in subcellular preparations of drosophila larvae with Leucine-U-14C and Uridine-2-14C respectively. The incorporation of leucine into proteins decreases progressively from crude homogenate to the microsome-free fraction in the same way as the degradation of the compounds. During RNA biosynthesis the incorporation of uridine increases from the crude homogenate up to mithocondria-free fraction and with Altosid is always below the test controls. The reduced incorporation of uridine into RNA and leucine into proteins in the presence of Altosid and Altozar indicates a genetic action of this compounds comparable to that of natural J.H. but slightly more efficient.
The chemical ionization mass spectrum of peroxyacetylnitrate, a major component of photochemical smog highly toxic to man and plants, was obtained using both methane and isobutane as reagent gas. The spectrum contains a [M + HI+ ion at m/e 122, intense fragment ions at m/e 43 [CH,CO]+ and m/e 46 [NO,]+, and less intense ions at m/e 61 [CH,ONO]+, m/e 77 [CH30NOz]+ and m/e 88 [+O=CCH,ONO] formed by internal rearrangements. These results confirm the commonly accepted structure for peroxyacetylnitrate, CH,(CO)OONO,.
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